Plasmodium berghei oocysts possess fatty acid synthesis and scavenging routes.
Plasmodium berghei oocysts possess fatty acid synthesis and scavenging routes.
复制标题
DOI：
10.1038/s41598-023-39708-z
复制
发表时间：
2023-08-05
影响因子：
4.6
通讯作者：
Dessens, Johannes T.
中科院分区：
文献类型：
--
作者：
Saeed, Sadia;Tremp, Annie Z.;Dessens, Johannes T.
疟疾寄生虫在其顶质体细胞器中通过细菌相关（II型）酶促途径进行脂肪酸合成（FAS）。在脊椎动物宿主中，红细胞外疟原虫阶段依赖于FAS，而红细胞内阶段依赖于从其环境中清除FA。在蚊子中，恶性疟原虫卵囊表达并依赖FAS酶来形成子孢子，但约氏疟原虫卵囊不表达也不依赖FAS酶，因此依赖FA清除来支持孢子生殖。在伯氏疟原虫中，FAS酶对于孢子生殖同样是消耗性的，表明它符合约氏疟原虫的情况。我们在这里显示，伯氏疟原虫，意想不到的是，在整个卵囊发育过程中表达FAS酶。这些发现表明，伯氏疟原虫可以利用FAS和FA清除，以最大限度地提高孢子生殖和传播，并且比以前假设的更类似于恶性疟原虫卵囊获得FA。卵囊在FAS和清除之间的转换能力可能是疟原虫与其蚊媒之间资源开发的非竞争关系的重要因素，这形成了疟原虫在昆虫和脊椎动物中的毒力。
Malaria parasites carry out fatty acid synthesis (FAS) in their apicoplast organelle via a bacterially related (type II) enzymatic pathway. In the vertebrate host, exoerythrocytic Plasmodium stages rely on FAS, whereas intraerythrocytic stages depend on scavenging FA from their environment. In the mosquito, P. falciparum oocysts express and rely on FAS enzymes for sporozoite formation, but P. yoelii oocysts do not express, nor depend on, FAS enzymes and thus rely on FA scavenging to support sporogony. In P. berghei, FAS enzymes are similarly expendable for sporogony, indicating it conforms to the P. yoelii scenario. We show here that P. berghei, unexpectedly, expresses FAS enzymes throughout oocyst development. These findings indicate that P. berghei can employ FAS alongside FA scavenging to maximise sporogony and transmission, and is more similar to P. falciparum than previously assumed with respect to FA acquisition by the oocyst. The ability of oocysts to switch between FAS and scavenging could be an important factor in the non-competitive relationship of resource exploitation between Plasmodium parasites and their mosquito vectors, which shapes parasite virulence both in the insect and vertebrate.
登录
查看更多内容
影响因子：
6.7
作者：
Shaw WR;Holmdahl IE;Itoe MA;Werling K;Marquette M;Paton DG;Singh N;Buckee CO;Childs LM;Catteruccia F
通讯作者：
Catteruccia F
影响因子：
9.8
作者：
Rono MK;Whitten MM;Oulad-Abdelghani M;Levashina EA;Marois E
通讯作者：
Marois E
影响因子：
4.4
作者：
Guttery DS;Pandey R;Ferguson DJ;Wall RJ;Brady D;Gupta D;Holder AA;Tewari R
通讯作者：
Tewari R
影响因子：
30.3
作者：
Yu M;Kumar TR;Nkrumah LJ;Coppi A;Retzlaff S;Li CD;Kelly BJ;Moura PA;Lakshmanan V;Freundlich JS;Valderramos JC;Vilcheze C;Siedner M;Tsai JH;Falkard B;Sidhu AB;Purcell LA;Gratraud P;Kremer L;Waters AP;Schiehser G;Jacobus DP;Janse CJ;Ager A;Jacobs WR Jr;Sacchettini JC;Heussler V;Sinnis P;Fidock DA
通讯作者：
Fidock DA
影响因子：
64.5
作者：
Stanway，Rebecca R.;Bushell，Ellen;Heussler，Volker T.
通讯作者：
Heussler，Volker T.