Separation and characterization of sulfonates in dissolved organic matter from industrial wastewater by solid phase extraction and high-resolution mass spectrometry
Separation and characterization of sulfonates in dissolved organic matter from industrial wastewater by solid phase extraction and high-resolution mass spectrometry
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DOI：
10.1007/s00216-022-04092-6
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发表时间：
2022-05
影响因子：
4.3
通讯作者：
Weiyi Deng;Chen He;Q. Shi;Haiyan Huang
中科院分区：
文献类型：
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作者：
Weiyi Deng;Chen He;Q. Shi;Haiyan Huang
电喷雾离子源（ESI）与高分辨质谱（HRMS）联用已成功用于工业废水中溶解性有机物（DOM）的分子组成表征。然而，这些废水中普遍存在磺酸盐，并且对ESI分析显示出较强的电离抑制，严重影响了其他DOM组分的表征。采用聚合物阴离子交换（PAX）固相萃取（SPE）技术，将石油炼制和焦化废水中的DOM分离为疏水中性、疏水酸性和疏水强酸，并采用负离子电喷雾傅立叶变换离子回旋共振质谱（ESI Fourier transform ion cyclotron resonance mass spectrometry，ESI-MS）进行分析。结果表明，PAX-SPE从废水中分离出磺酸盐，并使含氧化合物的分子表征成为可能，含氧化合物是废水中DOM的主要成分。对炼油废水和焦化废水中的磺酸盐进行了半定量分析，结果表明，炼油废水和焦化废水中的磺酸盐分别占总有机碳的11%和2%。PAX-SPE采用分步洗脱和高分辨率质谱分析，能够全面表征工业废水DOM的分子组成。该方法具有广泛的应用前景，其结果对了解工业废水中DOM的分子组成具有指导意义。
Electrospray ionization (ESI) source combined with high-resolution mass spectrometry (HRMS) has been successfully used to characterize the molecular composition of dissolved organic matter (DOM) in industrial wastewaters. However, sulfonates are commonly presenting in these wastewaters and showing strong ionization suppression for the ESI analysis, which seriously affects the characterization of other DOM components. In this paper, a novel method based on polymer anion exchange (PAX) solid phase extraction (SPE) was proposed to separate DOMs from petroleum refining and coal coking wastewaters into hydrophobic neutral, hydrophobic acids, and hydrophobic strong acids; the fractions were analyzed by negative-ion ESI Fourier transform ion cyclotron resonance mass spectrometry. The results show that PAX-SPE separated sulfonates from wastewaters and enabled the molecular characterization of oxygen-containing compounds, which are the major component of DOM in the wastewaters. Semi-quantitative analysis of sulfonates in the wastewaters was carried out and the results show that these compounds account for 11% and 2% in total organic carbon for the refinery and coal coking wastewaters, respectively. PAX-SPE with stepwise elution and followed by high-resolution mass spectrometry analysis enables a comprehensive characterization for the molecular composition of industrial wastewater DOM. The method has potential for extensive application and the results are instructive for the understanding of the molecular composition of DOM in industrial wastewaters.Graphical abstract