Fundamentals of protein separations: 50 years of nanotechnology, and growing.
Fundamentals of protein separations: 50 years of nanotechnology, and growing.
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DOI：
10.1146/annurev.anchem.1.031207.112912
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发表时间：
2008-06
影响因子：
8
通讯作者：
David A. Egas;M. Wirth
中科院分区：
文献类型：
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作者：
David A. Egas;M. Wirth
生物样品中蛋白质的分离一直被认为是一项重要而艰巨的奋进，对人类健康产生巨大影响。今天的蛋白质分离技术起源于20世纪50年代和60年代的早期纳米技术，方法包括免疫测定和其他亲和提取，电泳和色谱法。今天的不同之处在于需要在复杂的生物基质中解析和识别许多低丰度蛋白质。多维分离是规则，需要高速度，并且分离必须能够与质谱法一起用于蛋白质鉴定。结合联合收割机不同分离工具（包括识别、电泳和色谱）的混合方法利用了没有单一类别的分离可以解析生物基质中的蛋白质的事实。蛋白质分离代表了技术上的一个发展领域，从分子和纳米尺度的角度了解蛋白质分离的原理将使今天的研究人员能够发明明天的技术。
The separation of proteins in biology samples has long been recognized as an important and daunting endeavor that continues to have enormous impact on human health. Today's technology for protein separations has its origins in the early nanotechnology of the 1950s and 1960s, and the methods include immunoassays and other affinity extractions, electrophoresis, and chromatography. What is different today is the need to resolve and identify many low-abundance proteins within complex biological matrices. Multidimensional separations are the rule, high speed is needed, and the separations must be able to work with mass spectrometry for protein identification. Hybrid approaches that combine disparate separation tools (including recognition, electrophoresis, and chromatography) take advantage of the fact that no single class of separation can resolve the proteins in a biological matrix. Protein separations represent a developing area technologically, and understanding the principles of protein separations from a molecular and nanoscale viewpoint will enable today's researchers to invent tomorrow's technology.