CHANGES IN FREE FATTY-ACIDS, PHOSPHOLIPIDS, AND CHOLESTEROL FOLLOWING IMPACT INJURY TO THE RAT SPINAL-CORD (Retracted Article)
CHANGES IN FREE FATTY-ACIDS, PHOSPHOLIPIDS, AND CHOLESTEROL FOLLOWING IMPACT INJURY TO THE RAT SPINAL-CORD (Retracted Article)
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DOI：
10.1002/jnr.490230113
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发表时间：
1989-05-01
影响因子：
4.2
通讯作者：
FADEN, AI
中科院分区：
文献类型：
--
作者：
DEMEDIUK, P;DALY, MP;FADEN, AI
游离脂肪酸（FFA），磷脂，和胆固醇水平进行了测量脊髓样品从大鼠受到低（25克-厘米），中度（50克-厘米），或严重（100克-厘米）的冲击创伤的T10脊髓节段。所有程度的损伤导致创伤后总磷脂的早期（15分钟）下降;在中度和重度损伤的大鼠中，磷脂水平显著低于对照组长达3天，而在低损伤组中，磷脂在4小时内恢复到基线值。在所有损伤组中观察到胆固醇水平的快速和持续降低。严重创伤与FFA水平的双相增加相关：水平在创伤后5和15分钟升高，并在30分钟时下降;在1小时时观察到第二次升高，逐渐增加，在24小时达到最大值，然后在接下来的6天内下降。轻度和中度损伤导致早期总FFA增加相似;后期增加明显小于严重损伤组。在游离脂肪酸中，棕榈酸、硬脂酸、油酸、亚油酸、亚麻酸、花生四烯酸和二十二碳六烯酸显著增加。这些结果表明，创伤性脊髓损伤的结果在早期，短暂的，伤后膜磷脂水解，其幅度是相对独立的损伤的严重程度。更延迟和持续的脂质水解也发生在创伤后，其程度相对独立于损伤的严重程度。更延迟和持续的脂质水解也发生在创伤后，其程度与损伤的严重程度有关。
Free fatty acids (FFA), phospholipid, and cholesterol levels were measured in spinal cord samples from rats subjected to low (25 g-cm), moderate (50 g-cm), or severe (100 g-cm) impact trauma to the T10 spinal segment. All degrees of injury caused early (15 min) declines in total phospholipids after trauma; phospholipid levels remained significantly below controls in rats subjected to moderate and severe injuries for up to 3 days, whereas phospholipids had returned to baseline values by 4 hr in the low injury group. Rapid and persistent decreases in cholesterol levels were observed for all injury groups. Severe trauma was associated with biphasic increases in FFA levels: levels were elevated at 5 and 15 min post-trauma and had declined by 30 min; a second elevation was observed at 1 hr, progressively increasing to reach a maximum at 24 hr, before declining over the next 6 days. Low and moderate injuries caused similar early total FFA increases; later increases were significantly smaller than in the severely injured group. Among the free fatty acids, significant increases were observed in palmitate, stearate, oleate, linoleate, linolenate, arachidonate, and docosahexanoate. These findings indicate that traumatic spinal cord injury results in early, transient, postjury membrane phospholipid hydrolysis, the magnitude of which is relatively independent of the severity of injury. More delayed and sustained lipid hydrolysis also occurs after trauma, the magnitude of which is relatively independent of the severity of injury. More delayed and sustained lipid hydrolysis also occurs after trauma, the magnitude of which is related to the severity of injury.