Composition and content of glucosinolates in developing Arabidopsis thaliana
Composition and content of glucosinolates in developing Arabidopsis thaliana
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DOI：
10.1007/s004250100659
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发表时间：
2002-02-01
期刊：
影响因子：
4.3
通讯作者：
Halkier, BA
中科院分区：
文献类型：
--
作者：
Petersen, BL;Chen, SX;Halkier, BA
拟南芥各组织中芥子油苷的组成和含量。通过高效液相色谱法详细测定了哥伦比亚生态型从种子到抽苔植物的发育过程。将成熟植物的叶、根和茎的芥子油苷谱与绿色长角果和成熟种子的芥子油苷谱进行比较表明，大多数种子芥子油苷是在长角果中从头合成的。成熟种子与子叶的芥子油苷谱的比较表明，种子芥子油苷的主要部分保留在子叶中。通过含有放射性标记的对羟基苄基芥子油苷（p-OHBG）的种子的发芽来研究芥子油苷的周转率。种子中约 70% 的 [C-14]p-OHBG 含量在子叶期的幼苗中检测到，而在具有 6 至 8 叶莲座丛的幼苗中几乎检测不到 [C-14]p-OHBG。研究发现 p-OHBG 的更新与芥子油苷降解酶黑芥子酶的表达一致，黑芥子酶在子叶阶段幼苗中的水平非常低，但在发育后期的植物叶片中显着增加。这表明芥子油苷在发育过程中不断更新，而不仅仅是在组织破坏时。
The glucosinolate composition and content in various tissues of Arabidopsis thaliana (L.) Heynh. ecotype Columbia during development from seeds to bolting plants were determined in detail by high-performance liquid chromatography. Comparison of the glucosinolate profiles of leaves, roots and stems from mature plants with those of green siliques and mature seeds indicated that a majority of the seed glucosinolates were synthesized de novo in the silique. A comparison of the glucosinolate profile of mature seeds with that of cotyledons, indicated that a major part of seed glucosinolates was retained in the cotyledons. Turnover of glucosinolates was studied by germination of seeds containing radiolabelled p-hydroxybenzylglucosinolate (p-OHBG). Approximately 70% of the content of [C-14]p-OHBG in the seeds was detected in seedlings at the cotyledon stage and [C-14]p-OHBG was barely detectable in young plants with rosettes of six to eight leaves. The turnover of p-OHBG was found to coincide with the expression of the glucosinolate-degrading enzyme myrosinase, which was detectable at very low levels in seedlings at the cotyledon stage, but which dramatically increased in leaves from plants at later developmental stages. This indicates that there is a continuous turnover of glucosinolates during development and not only upon tissue disruption.