Evaluation of serum IgE in peach-allergic patients with systemic reaction by using recombinant Pru p 7 (gibberellin-regulated protein)
Evaluation of serum IgE in peach-allergic patients with systemic reaction by using recombinant Pru p 7 (gibberellin-regulated protein)
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DOI：
10.1016/j.aller.2018.02.007
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发表时间：
2018-09-01
影响因子：
1.8
通讯作者：
Kondo, Y.
中科院分区：
文献类型：
--
作者：
Mori, Y.;Okazaki, F.;Kondo, Y.
背景：脂质转移蛋白（LTP）是一种主要的水果过敏原。然而，最近发现桃过敏患者的全身反应不仅与LTP（Pru p 3）有关，而且与赤霉素调节蛋白（Pru p 7）有关。我们研究了重组Pru p 7（rPru p 7）在桃过敏诊断标准化中的潜在应用。方法：天然Pru p 7（nPru p 7）是从桃粗提物中用单克隆抗体亲和柱纯化的。克隆Prup 7的互补DNA并在大肠杆菌和巴斯德毕赤酵母中表达。用酶联免疫吸附试验（ELISA）检测了桃过敏患者血清免疫球蛋白（IG E）水平。大肠杆菌产物：结果：27例桃子过敏患者中，口服反应10例，全身反应17例。全身反应组nPrup 7阳性率为52%，口腔反应组为0%（P < 0.05）。在全身反应组中，nPrup 7与prup 7的反应性无显著性差异，而erPrup 7的反应性显著低于nPrup 7和prup 7（P < 0.05）。结论：在桃过敏的诊断中，prup 7与nPrup 7的反应性相当。(C)2018年SEICAP。出版社：Elsevier Espana，S.L.U. All rights reserved.
Background: Lipid transfer protein (LTP) is a major fruit allergen. It has, however, recently been revealed that the systemic reaction in peach-allergic patients is related not only to LTP (Pru p 3) but also to gibberellin-regulated protein (Pru p 7). We investigated recombinant Pru p 7 (rPru p 7) for its potential use in worldwide standardization for the diagnosis of peach allergy.Methods: Natural Pru p 7 (nPru p 7) was purified from peach crude extract using a monoclonal antibody affinity column. Complementary DNA for Pru p 7 was cloned and expressed in Escherichia coli and Pichia pastoris. Serum immunoglobutin (Ig) E in peach-allergic patients was examined by enzyme-linked immunosorbent assay (ELISA) using nPru p 7 and rPru p 7 (E. coli product: erPru p 7 and P pastoris product: prPru p 7).Results: Peach-allergic patients (n = 27) were diagnosed and categorized into oral reaction (n = 10) or systemic reaction (n= 17). The nPru p 7 positivity based on serum IgE levels was 52% in the systemic-reaction group and 0% in the oral-reaction group (P < 0.05). In the systemic reaction group, there was no significant difference in reactivity between nPru p 7 and prPru p 7, but the reactivity of erPru p 7 was significantly lower than those of nPru p 7 and prPru p 7 (P < 0.05).Conclusions: We found that prPru p 7 exhibited reactivity in ELISA comparable to that of nPru p 7 for the diagnosis of peach allergy with systemic reaction. (C) 2018 SEICAP. Published by Elsevier Espana, S.L.U. All rights reserved.