Dendritic cells are host cells for mycobacteria in vivo that trigger innate and acquired immunity
Dendritic cells are host cells for mycobacteria in vivo that trigger innate and acquired immunity
复制标题
DOI：
10.4049/jimmunol.168.3.1294
复制
发表时间：
2002-02-01
影响因子：
4.4
通讯作者：
Leclerc, C
中科院分区：
文献类型：
--
作者：
Jiao, XN;Lo-Man, R;Leclerc, C
在本研究中，我们研究了活菌感染小鼠后树突状细胞(DC)和巨噬细胞(Mphi)的感染和APC功能。实验用牛分枝杆菌卡介苗(BCG)或表达报告抗原的rBCG进行。小鼠感染DC和Mphi后，在体外分离的细胞上检测到免疫原肽/MHC-II类复合体的存在，IL-12p40的分泌也是如此。在这项研究中，我们发现DC是分枝杆菌的宿主细胞，我们在体内详细描述了在细菌感染的早期阶段，Mphi和DC在动员免疫方面的作用。引人注目的是，卡介苗在感染的前2周内存活下来，但在DC白细胞亚群中数量保持稳定。由于DC呈递的抗原迅速消失，这表明DC可能是分枝杆菌的储存库。
In the present study, we investigated in vivo the infection and APC functions of dendritic cells (DC) and macrophages (Mphi) after administration of live mvcobacteria to mice. Experiments were conducted with Mycobacterium bovis bacillus Calmette-Guerin (BCG) or a rBCG expressing a reporter Ag. Following infection of mice, DC and Mphi were purified and the presence of immunogenic peptide/MHC class II complexes was detected ex vivo on sorted cells, as was the secretion of IL-12 p40. We show in this study that DC is a host cell for mycobacteria, and we provide an in vivo detailed picture of the role of Mphi and DC in the mobilization of immunity during the early stages of a bacterial infection. Strikingly, BCG bacilli survive but remain stable in number in the DC leukocyte subset during the first 2 wk of infection. As Ag presentation by DC is rapidly lost, this suggests that DC may represent a bidden reservoir for mycobacteria.