A simple and sensitive assay for measuring very small volumes of microprinted solutions.
A simple and sensitive assay for measuring very small volumes of microprinted solutions.
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DOI：
10.4137/aci.s7827
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发表时间：
2011
期刊：
影响因子：
--
通讯作者：
Nishioka GM
中科院分区：
文献类型：
--
作者：
Sokolik CW;Walker AS;Nishioka GM
本研究描述了一种非常灵敏和简单的测定方法，用于测量<1 nL的小体积溶液。该分析利用Sandell-Kolthoff反应，其中黄色铈（IV）在砷（III）和催化量的碘离子的存在下被还原为无色铈（III）。该反应与Ce（IV）的还原速率和I−的存在量呈线性关系。典型的分析可以测量样品中10-100皮摩尔的碘化物。当I-取代标准生物缓冲液（如Tris缓冲盐水）中的氯离子时，该测定可用于确定微阵列中打印的溶液体积。
This study describes an extremely sensitive and simple assay to measure small volumes of solutions, <1 nL. The assay takes advantage of the Sandell-Kolthoff reaction in which yellow cerium(IV) is reduced to colorless cerium(III) in the presence of arsenic(III) and catalytic quantities of iodide ion. The reaction is linear with respect to the rate of Ce(IV) reduction and the quantity of I− present. Typical assays can measure 10–100 picomoles of iodide in a sample. When I− is substituted for chloride ion in standard biological buffers, such as Tris-buffered saline, the assay can be used to determine the volume of solution printed in a microarray.