Dynamic association of the mammalian insulator protein CTCF with centrosomes and the midbody.
Dynamic association of the mammalian insulator protein CTCF with centrosomes and the midbody.
复制标题
哺乳动物绝缘体蛋白 CTCF 与中心体和中体的动态关联。
DOI：
10.1016/j.yexcr.2003.11.015
复制
发表时间：
2004
影响因子：
3.7
通讯作者：
Renkawitz,Rainer
中科院分区：
文献类型：
--
作者：
Zhang,Ru;Burke,LesJ;Rasko,JohnEJ;Lobanenkov,Victor;Renkawitz,Rainer
CTCF是一种高度保守、普遍表达的DNA结合蛋白，具有广泛的基因调控能力。CTCF在细胞生长调控过程和表观遗传功能中发挥着重要作用。CTCF的异位表达会在细胞周期的多个时间点导致严重的细胞生长抑制，这表明必须严格监测CTCF的水平。我们对CTCF的亚细胞定位进行了详细的研究。有趣的是，我们观察到CTCF呈现动态的细胞周期依赖的分布。免疫荧光染色显示间期CTCF是一种核蛋白，主要排除在核仁之外。值得注意的是，在有丝分裂过程中，CTCF与中心体有关，特别是在中期到后期。在末期，CTCF从中心体解离，定位于中体和改造的核。生化分级进一步证实了CTCF与中心体和中体的联系。此外，CTCF的亚细胞部分显示出细胞周期和细胞器特异性的翻译后修饰，这表明CTCF在细胞周期的不同阶段发挥着不同的作用。
CTCF is a highly conserved, ubiquitously expressed DNA-binding protein that has widespread capabilities in gene regulation. CTCF plays important roles in cell growth regulatory processes and epigenetic functions. Ectopic expression of CTCF results in severe cell growth inhibition at multiple points within the cell cycle, indicating that CTCF levels must be stringently monitored. We have investigated the subcellular localization of CTCF in detail. Interestingly, we observe that CTCF shows a dynamic cell cycle-dependent distribution. Immunofluorescent staining reveals that in interphase CTCF is a nuclear protein, which is mainly excluded from the nucleolus. Strikingly, CTCF is associated with the centrosome during mitosis, especially from metaphase to anaphase. At telophase, CTCF dissociates from the centrosome and localizes to the midbody and the reformed nuclei. The association of CTCF with centrosomes and the midbody is further confirmed by biochemical fractionation. Moreover, subcellular fractions of CTCF show cell cycle and organelle-specific posttranslational modifications, suggesting different roles for CTCF at different stages of the cell cycle.