OBSERVATIONS ON EARLY EVENTS OF CADMIUM NECROSIS OF TESTIS
OBSERVATIONS ON EARLY EVENTS OF CADMIUM NECROSIS OF TESTIS
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DOI：
10.1002/ar.1091490104
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发表时间：
1964-01-01
影响因子：
--
通讯作者：
CHIQUOINE, A
中科院分区：
文献类型：
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作者：
CHIQUOINE, A
用光镜和电子显微镜对小鼠睾丸镉坏死的早期变化进行了研究，以鉴别1%氯化镉，剂量为0.1ml/10g体重，然后每小时固定睾丸进行光镜和电子显微镜观察。光镜下观察到的最早的改变包括肾小管间隙的水肿、血管充血和结缔组织间隙内颗粒沉淀物的增多。在电子显微镜下，最早的变化是在睾丸血管床的内皮细胞，包括吞噬前庭的发生率显着和迅速增加，表明血液和血管外间隙之间的液体交换增加。所有这些变化都发生在精液上皮细胞发生明显变化之前。在血管中观察到最早的变化这一事实表明，镉在睾丸坏死产生中的作用部位在血管床的内皮上。一系列比较动物(青蛙、鸽子、公鸡、螳螂、负鼠)对镉的毒性效应的敏感性也进行了调查。这些物种以及之前研究人员报道的其他物种的研究结果表明，镉坏死是具有阴囊睾丸的物种普遍存在的现象，而具有腹部睾丸的物种则不存在。负鼠是这种普遍性的一个例外。这些发现被讨论与睾丸的血液供应有关。
A light and electron microscopic study of the early changes which occur in cadmium necrosis of the testis of the mouse was made in an effort to identify 1% CdCl2 at a dosage of 0.1 ml/10 g body weight and then the testes were fixea for light and electron microscopy at various hourly intervals. The earliest changes which were appreciated by light microscopy consisted of an edema of the intertubular spaces, congestion of blood vessels, and an increased amount of granular precipitate in the connective tissue spaces. By electron microscopy the earliest changes were observed in the endothelium of the testicular vascular bed and consisted of a striking and rapid increase in the prevalence of pinocytotic vesticles suggesting an increased interchange of fluid between the blood and extravascular spaces. All of these changes preceded any appreciable alteration in the cells of the seminal epithelium. The fact that the earliest alterations are observed in blood vessels suggests that the site of action of cadmium in the production of testicular necrosis is upon the endothelium of the vascular bed. A comparative series of animals (frog, pigeon, rooster, armadillo, opossum) was also investigated as to their susceptibility to the toxic effects of cadmium. The results from these species, and other species reported by previous investigators, suggest the generality that cadmium necrosis is a phenomenon common to species possessing scrotal testes and absent from those possessing abdominal testes. The opossum is an exception to this generality. These findings are discussed in relation to the blood supply of the testis.