Functional analysis of retrotransposons in pea.
Functional analysis of retrotransposons in pea.
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DOI：
10.1093/oxfordjournals.pcp.a029625
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发表时间：
1999
影响因子：
4.9
通讯作者：
H. Kato;P. Sriprasertsak;H. Seki;Y. Ichinose;T. Shiraishi;T. Yamada
中科院分区：
文献类型：
--
作者：
H. Kato;P. Sriprasertsak;H. Seki;Y. Ichinose;T. Shiraishi;T. Yamada
豌豆植物主动防御基因(PSPAL2和PSCHS1)的5‘上游区域与部分Copia型反转录转座子具有显著的核苷酸序列同源性。为了鉴定豌豆中的反转录转座子，用聚合酶链式反应(PCR)从豌豆悬浮培养细胞原生质体中扩增出逆转录酶序列(PSR)。根据其核苷酸序列的相似性，PSR基因至少可分为8个亚组(A-H)。用真菌激发子处理黄化的豌豆上胚轴在30min内可诱导出一个PSRC亚群，而豌豆悬浮培养细胞的原生质体几乎不能诱导出PSRC亚群，而PsrA和PsrB亚群则被原生质体高度诱导。有趣的是，由真菌激发子诱导的位于PSCHS1 5‘-上游区域的反转录转座子序列属于PSRC亚群。反转录转座子可能在基因重组和基因表达优化中发挥重要作用，以响应各种环境刺激，如植物病原菌的攻击或原生质体的攻击。
The 5'-upstream regions of the plant active defense genes in pea (PSPAL2 and PSCHS1) exhibit significant nucleotide sequence identity to part of a copia-type retrotransposon. To characterize the retrotransposon in pea putative reverse transcriptase sequences (Psr) were amplified by PCR from cDNA prepared from protoplasts derived from pea suspension cultured cells. Psr genes can be classified into at least eight subgroups (A-H) according to their nucleotide sequence similarities. A subgroup PsrC was induced by the treatment of etiolated pea epicotyls with fungal elicitor within 30 min but was hardly induced by protoplasting of pea suspension cultured cells, whereas subgroups of PsrA and PsrB were highly induced by protoplasting. Interestingly, the retrotransposon sequence present at the 5'-upstream region of PSCHS1 which was induced by the treatment with fungal elicitor belonged to subgroup PsrC. The retrotransposon might play an important role in reorganization of the genes and optimization of the gene expression in response to various environmental stimuli such as an attack by phytopathogens or protoplasting.