INVITRO PRODUCTION OF REICHERT MEMBRANE BY MOUSE EMBRYO-DERIVED PARIETAL ENDODERM CELL-LINES
INVITRO PRODUCTION OF REICHERT MEMBRANE BY MOUSE EMBRYO-DERIVED PARIETAL ENDODERM CELL-LINES
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DOI：
10.1016/0014-4827(90)90005-u
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发表时间：
1990-12-01
影响因子：
3.7
通讯作者：
DZIADEK, M
中科院分区：
文献类型：
--
作者：
FOWLER, KJ;MITRANGAS, K;DZIADEK, M
我们报告了8个独立的细胞系从植入前小鼠胚胎，其中有壁内胚层表型的分离。当作为聚集体生长时，这些细胞系产生大量的基底膜基质，其含有层粘连蛋白、巢蛋白、硫酸乙酰肝素蛋白聚糖、胶原IV和BM-40。所有八种细胞系的生物合成谱与体内合成Reichert膜的壁内胚层细胞非常相似。壁内胚层细胞系（PEC系）产生的基质结构在对蛋白水解降解的敏感性方面比Engelbreth-Holm-Swarm（EHS）肿瘤更接近Reichert膜。由于这些细胞系产生大量的基底膜，它们将用于具有EHS肿瘤产生的基底膜的Reichert膜基质的结构和功能区室。
We report the isolation of eight independent cell lines from preimplantation mouse embryos, which have a parietal endoderm phenotype. When grown as aggregates, these cell lines produce large amounts of a basement membrane matrix, that contains laminin, nidogen, heparan sulfate proteoglycan, collagen IV, and BM-40. The biosynthetic profiles of all eight cell lines are very similar to parietal endoderm cells in vivo which synthesize Reichert''s membrane. The structure of the matrix produced by the parietal endoderm cell lines (PEC lines) resembles more closely Reichert''s membrane than the Engelbreth-Holm-Swarm (EHS) tumor in susceptibility to proteolytic degradation. Since these cell lines produce large quantities of basement membrane they will be useful for structural and functional compartment of a Reichert''s membrane matrix with the basement membrane produced by the EHS tumor.