Platelet-rich plasma contains high levels of platelet-derived growth factor and transforming growth factor-β and modulates the proliferation of periodontally related cells in vitro
Platelet-rich plasma contains high levels of platelet-derived growth factor and transforming growth factor-β and modulates the proliferation of periodontally related cells in vitro
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DOI：
10.1902/jop.2003.74.6.849
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发表时间：
2003-06-01
影响因子：
4.3
通讯作者：
Yoshie, H
中科院分区：
文献类型：
--
作者：
Okuda, K;Kawase, T;Yoshie, H
背景资料：富血小板血浆（PRP）是血浆的一部分，其中血小板衍生生长因子（PDGF）和转化生长因子-P（TGF-β）被认为是浓缩的。局部应用PRP上调细胞活性并随后促进体内牙周再生是合理的。然而，这些生长因子在PRP的浓度还没有具体确定和PRP在细胞和分子水平的生物学效应尚未determined.Methods：PRP从20名健康受试者通过离心从血浆中制备。立即使用酶联免疫吸附测定（ELISA）试剂盒对这些PRP制剂进行PDGF-AB和TGF-β 1评价。PRP制剂的生物学效应进行了评价成骨细胞，上皮细胞，成纤维细胞和牙周韧带细胞。通过计数细胞数或评价5 -溴脱氧尿苷（BrdU）掺入来评价细胞促有丝分裂活性。结果：在PRP制剂中，血小板被浓缩至70.9 × 10（4）个细胞/穆尔（未浓缩血浆的283.4%）。PDGF-AB和TGF-β 1的水平也分别浓缩至182.0 ng/ml（440.6%）和140.9 ng/ml（346.6%）。散点图显示血小板计数和这些生长因子水平之间存在显著相关性。富血小板血浆刺激成骨细胞DNA合成和细胞分裂（对照组的138%），同时下调碱性磷酸酶，但抑制上皮细胞分裂（对照组的80%）。PRP还刺激牙龈成纤维细胞和牙周膜cells.Conclusions的DNA合成：这些数据表明，PDGF-AB和TGF-β 1是高度集中的PRP制剂。这表明PRP以细胞类型特异性的方式调节细胞增殖，类似于用TGF-β 1观察到的方式。由于相关细胞类型的同步行为被认为是成功的牙周再生所必需的，因此进一步表明这些细胞类型特异性的作用可能有利于牙周再生治疗。
Background: Platelet-rich plasma (PRP) is a fraction of plasma, in which platelet-derived growth factor (PDGF) and transforming growth factor-P (TGF-beta) are thought to be concentrated. It is plausible that topically-applied PRP up-regulates cellular activity and subsequently promotes periodontal regeneration in vivo. However, the concentrations of these growth factors in PRP have not been specifically determined and the biological effects of PRP at the cellular and molecular levels have not been determined.Methods: PRP obtained from 20 healthy subjects was prepared from plasma by centrifugation. These PRP preparations were immediately subjected to an evaluation for PDGF-AB and TGF-beta1 using enzyme-linked immunosorbent assay (ELISA) kits. The biological effects of the PRP preparations were evaluated on osteoblastic, epithelial, fibroblastic, and periodontal ligament cells. Cellular mitogenic activity was evaluated by counting cell numbers or evaluating 5 - bromodeoxyuridine (BrdU) incorporation. Expression of alkaline phosphatase (ALP) was immunocytochemically evaluated.Results: In the PRP preparations, platelets were concentrated up to 70.9 x 10(4) cells/mul (283.4% of the unconcentrated plasma). The levels of PDGF-AB and TGF-beta1 were also concentrated up to 182.0 ng/ml (440.6%) and 140.9 ng/ml (346.6%), respectively. Scatter plots revealed significant correlations between platelet counts and levels of these growth factors. PRP stimulated osteoblastic DNA synthesis and cell division (138% of control), with simultaneous down-regulation of ALP, but suppressed epithelial cell division (80% of control). PRP also stimulated DNA synthesis in gingival fibroblasts and periodontal ligament cells.Conclusions: These data demonstrated that both PDGF-AB and TGF-beta1 were highly concentrated in the PRP preparations. It is suggested PRP modulates cell proliferation in a cell type-specific manner similar to what has been observed with TGF-beta1. Since synchronized behavior of related cell types is thought to be required for successful periodontal regeneration, it is further suggested these cell type-specific actions may be beneficial for periodontal regenerative therapy.