MOLECULAR ANALYSIS OF ENTEROTOXIGENIC, SHIGATOXIGENIC AND ENTEROAGGREGATIVE ESCHERICHIA COLI STRAINS ISOLATED FROM SUCKLING PIGLETS WITH DIARRHOEA BY THE USE OF PULSED-FIELD ELECTROPHORESIS
MOLECULAR ANALYSIS OF ENTEROTOXIGENIC, SHIGATOXIGENIC AND ENTEROAGGREGATIVE ESCHERICHIA COLI STRAINS ISOLATED FROM SUCKLING PIGLETS WITH DIARRHOEA BY THE USE OF PULSED-FIELD ELECTROPHORESIS
复制标题
利用脉冲场电泳对从腹泻哺乳仔猪中分离出的肠毒素、志贺毒素和肠聚集性大肠杆菌菌株进行分子分析
DOI：
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发表时间：
2004
期刊：
影响因子：
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通讯作者：
J. Osek
中科院分区：
文献类型：
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作者：
M. Weiner;J. Dacko;J. Osek
在 XbaI 核酸内切酶消化染色体 DNA 后，使用脉冲场凝胶电泳 (PFGE) 方法确定从患有新生儿腹泻的仔猪中分离的表达不同或相同菌毛/毒性毒力标记的大肠杆菌菌株之间的遗传关系。根据其毒力特性，这些菌株被分为产肠毒素（ETEC）、产志贺毒素（STEC）和肠聚集性（EAEC）大肠杆菌组。 PFGE 后，使用 Dice 系数和算术平均分析的未加权配对方法从波段匹配矩阵创建树状图。对所有测试分离株的遗传分析表明，具有相同毒力相关因子的细菌可以分为不同的克隆群。另一方面，具有不同毒力特性的两对 EAEC 分离株产生了相同的 PFGE 图谱。获得的结果显示了源自腹泻哺乳仔猪的不同大肠杆菌菌株之间分子关系的初步流行病学信息。
Genetic relationship between Escherichia coli strains expressing different or the same fimbrial/toxic virulence markers and isolated from piglets affected with neonatal diarrhoea were determined using the pulsed field gel electrophoresis (PFGE) method after XbaI endonuclease digestion of chromosomal DNA. The strains were classified as enterotoxigenic (ETEC), shigatoxigenic (STEC) and enteroaggregative (EAEC) groups of E. coli according to their virulence properties. After PFGE, the dendrograms were created from a matrix of band matching using the Dice coefficient and the unweighted pair group method with arithmetic mean analysis. The genetic analysis of all isolates tested revealed that bacteria possessing the same virulenceassociated factors can be classified as different clonal groups. On the other hand, two pairs of EAEC isolates with different virulence properties generated identical PFGE profiles. The obtained results show preliminary epidemiological information about the molecular relationship between diverse E. coli strains originated from suckling piglets with diarrhoea.