Immunosuppressive substances in Aspergillus fumigatus culture filtrate
Immunosuppressive substances in Aspergillus fumigatus culture filtrate
复制标题
烟曲霉培养滤液中的免疫抑制物质
DOI：
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发表时间：
2003
影响因子：
2.2
通讯作者：
T. Kuriyama
中科院分区：
文献类型：
--
作者：
A. Watanabe;K. Kamei;T. Sekine;M. Waku;K. Nishimura;M. Miyaji;T. Kuriyama
摘要侵袭性曲霉病已成为临床上的一个严重问题，但致病因素尚未完全阐明。 为了鉴定和分离该真菌产生的免疫抑制物质，对培养物进行了生物活性评价，并对培养物进行了分析。评估了不同烟曲霉菌株的培养物对人多形核白细胞和小鼠巨噬细胞的影响。为了评估它们在体内的活性，还研究了它们对被真菌感染的小鼠的存活的影响。随后，通过气相色谱-质谱法分析培养物的组成。分析表明，培养物中含有浓度为3 - 4 μg/ml的胶胶毒素和一些其他未鉴定的化合物。其生物活性与胶胶毒素相似。真菌培养滤液降低了感染小鼠的存活率，但滤液本身并不导致小鼠死亡。然而，所有的生物活性都不能由胶粘毒素本身来解释。这些结果表明，培养细胞中的胶质毒素可能是部分免疫抑制活性的原因，但A.烟曲霉毒素以加和或协同的方式有助于真菌的毒力。
Abstract Invasive aspergillosis has become a serious problem in clinical practice, but the actual factor that confers virulence on the fungus has not been thoroughly elucidated. To identify and isolate the immunosuppressive substances produced by the fungus, the bioactivity of culture filtrates was assessed, and analyses of the culture filtrates were carried out. Culture filtrates from different strains of Aspergillus fumigatus were assessed for their effect on human polymorphonuclear leukocytes and murine macrophages. To assess their activities in vivo, their effect on the survival of mice infected by the fungus was also studied. Subsequently, the composition of the culture filtrates was analyzed by gas chromatography-mass spectrometry. The analyses revealed that the culture filtrates contained gliotoxin at concentrations of 3 to 4 μg/ml, and some other unidentified compounds. The bioactivities of the culture filtrates were similar to those of gliotoxin. The fungal culture filtrate reduced the survival of infected mice, but the filtrate itself did not cause the death of mice. However, all the bioactivities could not be accounted for by gliotoxin itself. These results indicate that gliotoxin in the culture filtrates may be responsible for part of the immunosuppressive activity, but some other components produced by A. fumigatus contribute, in an additive or synergistic manner, to the virulence of the fungus.
影响因子：
4.4
作者：
L. Arruda;B. Mann;M. Chapman
通讯作者：
L. Arruda;B. Mann;M. Chapman