New molecular variants of epsilon and beta IncP-1 plasmids are present in estuarine waters
New molecular variants of epsilon and beta IncP-1 plasmids are present in estuarine waters
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DOI：
10.1016/j.plasmid.2011.11.002
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发表时间：
2012-05-01
期刊：
影响因子：
2.6
通讯作者：
Correia, A.
中科院分区：
文献类型：
--
作者：
Oliveira, C. S.;Lazaro, B.;Correia, A.
在这项工作中，我们研究了葡萄牙河口广泛宿主质粒的存在。以恶臭假单胞菌KT2442为模式受体菌，在四环素和汞的双亲配对中进行耐药表型筛选。结果，通过对trfA基因的PCR扩增，发现7个转接子携带来自IncP-1组的广宿主质粒。序列分析证实从β-1亚群中分离到4个质粒，并将3个分配给最近描述的c亚群。据我们所知，这是关于在河口水域中检测和分离IncP-1β和epsilon质粒的第一份报告。此外，研究表明，尽管检索到的质粒在系统发育上与先前鉴定的质粒(如pB10和pKJK5)接近，但它们构成了新的分子变体。(C)2011 Elsevier Inc.保留所有权利。
In this work the presence of broad-host-plasmids in an estuary in Portugal has been investigated. Pseudomonas putida KT2442 was used as model recipient bacteria in biparental matings with tetracycline and mercury to select for resistance phenotypes. As a result, 7 transconjugants were shown to carry broad-host-plasmids from the IncP-1 group, as seen by PCR amplification of the trfA gene. Sequence analysis confirmed the isolation of 4 plasmids from beta-1 subgroup and 3 assigned to the recently described c subgroup. To our knowledge this is the first report concerning the detection and isolation of IncP-1 beta and epsilon plasmids in estuarine waters. Moreover it is shown that, even though the retrieved plasmids are phylogenetically close to previously characterized plasmids, such as pB10 and pKJK5, respectively, they constitute new molecular variants. (C) 2011 Elsevier Inc. All rights reserved.