Protoplast culture and plant regeneration of several species in the genus Dianthus
Protoplast culture and plant regeneration of several species in the genus Dianthus
复制标题
石竹属几种植物的原生质体培养及植株再生
DOI：
10.1007/bf00235070
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发表时间：
1992
影响因子：
6.2
通讯作者：
M. Mii
中科院分区：
文献类型：
--
作者：
M. Nakano;M. Mii
在相同条件下对17个石竹属品种进行原生质体分离、培养和芽再生研究。这些包括D.石竹、D. chinensis、D. barbatus、D. plumarius、D. superbus 和 D. japonicusas 以及种间杂交品种（D. caryophyllus x D. chinensis 和 D. chinensis x D. barbatus）。在所有品种中，从无菌芽培养物的叶子中以高产率分离出存活的原生质体，并且其中一些原生质体分裂并形成菌落。然而，不同物种之间的芽再生频率明显不同。高频芽再生获得自D。中华草和种间杂交品种，而其他物种仅获得低频率或没有芽再生。
Seventeen cultivars belonging to the genusDianthuswere examined for protoplast isolation, culture and shoot regeneration under the same conditions. These includedD. caryophyllus, D. chinensis, D. barbatus, D. plumarius, D. superbusandD. japonicusas well as interspecific hybrid cultivars (D. caryophyllus x D. chinensisandD. chinensis x D. barbatus). In all cultivars, viable protoplasts were isolated at high yields from leaves of axenic shoot cultures and some of these protoplasts divided and formed colonies. However, shoot regeneration frequencies were markedly different among the species. High frequency shoot regeneration was obtained fromD. chinensisand interspecific hybrid cultivars, while only low frequency or no shoot regeneration was obtained from other species.