Direct One-Step Immobilization of Glucose Oxidase in Well-Ordered Mesostructured Silica Using a Nonionic Fluorinated Surfactant
Direct One-Step Immobilization of Glucose Oxidase in Well-Ordered Mesostructured Silica Using a Nonionic Fluorinated Surfactant
复制标题
DOI：
10.1021/cm048033r
复制
发表时间：
2005-02
影响因子：
8.6
通讯作者：
J. Blin;C. Gérardin;C. Carteret;L. Rodehüser;C. Selve;M. Stébé
中科院分区：
文献类型：
--
作者：
J. Blin;C. Gérardin;C. Carteret;L. Rodehüser;C. Selve;M. Stébé
这项工作描述了葡萄糖氧化酶（GOD）在介观结构二氧化硅中的固定化。通过直接一步固定法将酶整合到二氧化硅框架中。 SAXS 和氮气吸附-解吸分析获得的结果清楚地表明，回收材料的通道排列取决于 GOD 负载。事实上，当水热处理在60℃下进行2天时，如果GOD浓度低于每毫升胶束溶液3.2毫克，则可以获得良好有序的材料，并且较高的负载量会导致虫洞状结构的形成。通过荧光和 FTIR 光谱揭示了固定效率。看来可以掺入基质中的 GOD 最大负载量约为 11 wt%。结果还表明表面活性剂起到了成孔剂的作用。最后，我们证明了捕获的酶保持了其活性。
This work describes the immobilization of glucose oxidase (GOD) in mesostructured silica. The enzyme is incorporated into the silica framework via a direct one-step immobilization method. Results obtained by SAXS and nitrogen adsorption−desorption analysis clearly show that the channel arrangement of the recovered materials depends on the GOD loading. Indeed, when the hydrothermal treatment is performed at 60 °C for 2 days, well-ordered materials are obtained if the GOD concentration is lower than 3.2 mg per mL of micellar solution, and higher loading leads to the formation of wormhole-like structures. The efficiency of the immobilization was revealed by fluorescence and FTIR spectroscopy. It appears that there is a maximum loading of GOD, about 11 wt %, that can be incorporated into the matrix. Results also show that the surfactant plays the role of a pore-forming agent. Finally, we have shown that the entrapped enzyme maintains its activity.