Morpho-dynamic changes of mitochondria during ageing of human endothelial cells
Morpho-dynamic changes of mitochondria during ageing of human endothelial cells
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DOI：
10.1016/j.mad.2005.03.002
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发表时间：
2005-06-01
影响因子：
5.3
通讯作者：
Bereiter-Hahn, J
中科院分区：
文献类型：
--
作者：
Jendrach, M;Pohl, S;Bereiter-Hahn, J
在许多培养的真核细胞中，线粒体形态受线粒体融合和裂变的调节。裂变和融合事件之间需要一个严格控制的平衡，以确保正常的线粒体和细胞功能。在衰老过程中，线粒体在功能和形态水平上发生了显著的变化。衰老对线粒体融合和裂变的影响以及改变的裂变和融合活动的后果仍然未知，尽管衰老的理论模型考虑了这些过程的重要性。人类。脐静脉内皮细胞（HUVECs）已被建立为细胞培养模型，以跟踪衰老过程中线粒体的活性和功能障碍。老龄HUVECs和有丝分裂后HUVECs的线粒体整体形态和精细结构发生明显改变，线粒体膜电位丧失。同时，观察到完整线粒体DNA （mtDNA）减少。测定活细胞线粒体的裂变和融合活性。老年HUVECs线粒体的融合和裂变活性均显著下降，表明这些过程对衰老很敏感，可能导致衰老过程中受损线粒体的积累。©2005爱思唯尔爱尔兰公司版权所有。
Mitochondrial morphology is regulated in many cultured eukaryotic cells by fusion and fission of mitochondria. A tightly controlled balance between fission and fusion events is required to ensure normal mitochondrial and cellular functions. During ageing, mitochondria are undergoing significant changes on the functional and morphological level. The effect of ageing on fusion and fission of mitochondria and consequences of altered fission and fusion activity are still unknown although theoretical models on ageing consider the significance of these processes. Human. umbilical vein endothelial cells (HUVECs) have been established as a cell culture model to follow mitochondrial activity and dysfunction during the ageing process. Mitochondria of old and postmitotic HUVECs showed distinct alterations in overall morphology and fine structure, and furthermore, loss of mitochondrial membrane potential. In parallel, a decrease of intact mitochondrial DNA (mtDNA) was observed. Fission and fusion activity of mitochondria were quantified in living cells. Mitochondria of old HUVECs showed a significant and equal decrease of both fusion and fission activity indicating that these processes are sensitive to ageing and could contribute to the accumulation of damaged mitochondria during ageing. © 2005 Elsevier Ireland Ltd. All rights reserved.