Adrenalectomy of the obese Zucker rat: effects on the feeding response to enterostatin and specific mRNA levels.
Adrenalectomy of the obese Zucker rat: effects on the feeding response to enterostatin and specific mRNA levels.
复制标题
肥胖 Zucker 大鼠的肾上腺切除术：对肠抑素和特定 mRNA 水平的喂养反应的影响。
DOI：
10.1152/ajpregu.1993.265.1.r21
复制
发表时间：
1993
期刊：
影响因子：
--
通讯作者：
Bray,GA
中科院分区：
文献类型：
--
作者：
Okada,S;Onai,T;Kilroy,G;York,DA;Bray,GA
本研究探讨了肾上腺切除对瘦肉型（fa/？）和遗传性肥胖（FA/FA）大鼠。肾上腺切除术减少了肥胖大鼠的体重增加和食物摄入。肠抑素抑制肥胖大鼠高脂饮食的摄入，但对瘦大鼠无抑制作用。肾上腺切除术减少了所有大鼠的摄食量，并取消了肥胖组对肠抑素的反应。肥胖大鼠的辅脂酶mRNA水平较低，但肾上腺切除术后恢复正常。对外源性肠抑素的反应能力可能与肽的低水平产生有关。观察肾上腺切除对棕色脂肪组织解偶联蛋白（UCP）mRNA和β 3肾上腺素能受体（β 3 AR）mRNA表达的影响。北方印迹分析显示，肥胖大鼠的UCP mRNA和β 3-AR mRNA水平较低，通过肾上腺切除术恢复到或接近瘦大鼠的正常水平。肾上腺切除对瘦大鼠的mRNA水平无显著影响。
The effects of adrenalectomy on the feeding response to enterostatin and the mRNA levels of its parent protein, pancreatic colipase, have been investigated in lean (fa/?) and genetically obese (fa/fa) rats. Adrenalectomy reduced body weight gain and food intake of obese rats. Enterostatin inhibited the intake of high-fat diet in obese rats but not in lean rats. Adrenalectomy reduced food intake of all rats and abolished the response to enterostatin in the obese group. Obese rats had low levels of colipase mRNA, but these were normalized after adrenalectomy. The ability to respond to exogenous enterostatin is possibly linked to low levels of production of the peptide. The effects of adrenalectomy on brown adipose tissue uncoupling protein (UCP) mRNA and beta 3-adrenergic receptor (beta 3-AR) mRNA were also investigated. Northern blot analysis showed low levels of both UCP mRNA and beta 3-AR mRNA in obese rats that were restored to or toward the normal levels of lean rats by adrenalectomy. Adrenalectomy had no significant effects on mRNA levels in lean rats.