SARS-CoV2 in public spaces in West London, UK during COVID-19 pandemic.
SARS-CoV2 in public spaces in West London, UK during COVID-19 pandemic.
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DOI：
10.1136/bmjresp-2022-001574
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发表时间：
2023-05
影响因子：
4.1
通讯作者：
中科院分区：
文献类型：
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作者：
通过气溶胶传播SARS-CoV 2被认为是距离>2米的重要传播方式，特别是在室内。我们确定是否可以在封闭/半封闭公共空间的空气中检测到SARS-CoV 2。于2021年3月至2021年12月期间，在COVID-19疫情限制措施于一段时间封城后放宽期间，我们使用全悬浮及大小隔离颗粒物（PM）采样器在医院病房及等候区、公共交通工具、大学校园及西伦敦一所小学检测SARS-CoV 2。我们收集了207份样本，其中20份（9.7%）为SARS-CoV 2阳性。阳性样本是从医院病人等候区、使用固定采样器治疗COVID-19患者的医院病房以及使用个人采样器从伦敦地铁的火车车厢收集的。平均病毒浓度在医院急诊等候区的429 500份/m3和其他地区发现的更常见的164 000份/m3之间。与PM10和PM 1相比，PM2.5组分中来自PM采样器的阳性样品更频繁。所有采集的样品在Vero细胞上培养均显示阴性结果。在伦敦COVID-19大流行期间部分开放期间，我们在医院等候区和病房以及伦敦地铁车厢的空气中检测到SARS-CoV 2 RNA。需要更多的研究来确定在空气中检测到的SARS-CoV 2的传播潜力。
Spread of SARS-CoV2 by aerosol is considered an important mode of transmission over distances >2 m, particularly indoors. We determined whether SARS-CoV2 could be detected in the air of enclosed/semi-enclosed public spaces. Between March 2021 and December 2021 during the easing of COVID-19 pandemic restrictions after a period of lockdown, we used total suspended and size-segregated particulate matter (PM) samplers for the detection of SARS-CoV2 in hospitals wards and waiting areas, on public transport, in a university campus and in a primary school in West London. We collected 207 samples, of which 20 (9.7%) were positive for SARS-CoV2 using quantitative PCR. Positive samples were collected from hospital patient waiting areas, from hospital wards treating patients with COVID-19 using stationary samplers and from train carriages in London underground using personal samplers. Mean virus concentrations varied between 429 500 copies/m3 in the hospital emergency waiting area and the more frequent 164 000 copies/m3 found in other areas. There were more frequent positive samples from PM samplers in the PM2.5 fractions compared with PM10 and PM1. Culture on Vero cells of all collected samples gave negative results. During a period of partial opening during the COVID-19 pandemic in London, we detected SARS-CoV2 RNA in the air of hospital waiting areas and wards and of London Underground train carriage. More research is needed to determine the transmission potential of SARS-CoV2 detected in the air.