Expression of prolactin receptors in normal, benign, and malignant breast tissue: an immunohistological study
Expression of prolactin receptors in normal, benign, and malignant breast tissue: an immunohistological study
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DOI：
10.1136/jcp.54.12.956
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发表时间：
2001-12-01
影响因子：
3.4
通讯作者：
Shousha, S
中科院分区：
文献类型：
--
作者：
Gill, S;Peston, D;Shousha, S
目的：催乳素在正常乳腺上皮细胞的增殖和分化中起重要作用，并可能在乳腺癌的发生发展中起重要作用。催乳素的作用是由其受体介导的；因此，这种受体表达的改变在研究乳腺癌生物学中可能是重要的。本研究旨在比较泌乳素受体在正常、良性和恶性乳腺组织中的表达。材料/方法：采用单克隆抗体B6.2和亲和素-生物素免疫过氧化物酶技术，对102例乳腺活检组织（女性93例，男性9例）石蜡包埋切片中催乳素受体的表达进行了研究。6例活检正常，34例为良性，62例为恶性。结果：正常情况下，催乳素受体阳性仅见于导管和腺泡上皮细胞的腔内边界。在大多数良性病变中，可见不同程度的管腔和细胞质染色。大汗液化生和常规导管上皮增生的细胞多为阴性。在恶性病例中，染色模式主要是细胞质和异质性。在女性的59例癌中，有41例显示出10-100%的肿瘤细胞呈一定程度的阳性。当使用H评分系统仅考虑后一受体评分超过100/300的病例时，发现催乳素受体与雌激素受体染色之间存在显著的直接相关性（p = 0.0207）。催乳素受体和孕激素受体、患者年龄、肿瘤大小、肿瘤分级或腋窝淋巴结状态之间没有相关性。结论：泌乳素受体似乎在正常、良性和恶性乳腺上皮细胞的不同细胞部位表达。该受体在超过三分之二的女性乳腺癌中表达，这表明它可能在该疾病的发病机制中发挥作用。阳性与组织切片中雌激素受体的中度和强烈染色相关，但与其他预后因素无关。
Aims-Prolactin plays an important role in the proliferation and differentiation of normal breast epithelium, and possibly in the development of breast carcinoma. The effects of prolactin are mediated by its receptor; thus, alteration in the expression of this receptor could be important in studying the biology of breast cancer. This investigation was aimed at comparing the expression of prolactin receptors in normal, benign, and malignant breast tissue.Material/Methods-The expression of prolactin receptors was studied in paraffin wax embedded sections of 102 breast biopsies (93 female and nine male), using the monoclonal antibody B6.2, and the avidin-biotin immunoperoxidase technique. Six biopsies were normal, 34 had benign lesions, and 62 were malignant.Results-In normal cases, prolactin receptor positivity was seen only on the luminal borders of the epithelial cells lining ducts and acini. In most benign lesions, variable degrees of luminal and cytoplasmic staining were seen. Cells showing apocrine metaplasia and florid regular ductal epithelial hyperplasia were mostly negative. In malignant cases, the staining pattern was mostly cytoplasmic and heterogeneous. Forty one of the 59 carcinomas in women showed a degree of positivity involving 10-100% of the tumour cells. A significant direct correlation was found between prolactin receptor and oestrogen receptor staining when only cases that scored more than 100/300 for the latter receptor, using the H scoring system, were considered (p = 0.0207). No correlation was found between prolactin receptors and progesterone receptors, patient's age, tumour size, tumour grade, or axillary lymph node status.Conclusions-Prolactin receptors seem to be expressed at different cellular sites in normal, benign, and malignant breast epithelial cells. The receptor is expressed in more than two thirds of female breast carcinomas, suggesting that it may play a role in the pathogenesis of the disease. The positivity is correlated with moderate and strong staining for oestrogen receptors in tissue sections, but not with other prognostic factors.