HUMAN RO RIBONUCLEOPROTEIN-PARTICLES - CHARACTERIZATION OF NATIVE STRUCTURE AND STABLE ASSOCIATION WITH THE LA POLYPEPTIDE
HUMAN RO RIBONUCLEOPROTEIN-PARTICLES - CHARACTERIZATION OF NATIVE STRUCTURE AND STABLE ASSOCIATION WITH THE LA POLYPEPTIDE
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DOI：
10.1172/jci114551
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发表时间：
1990-04-01
影响因子：
15.9
通讯作者：
CRAFT, J
中科院分区：
文献类型：
--
作者：
BOIRE, G;CRAFT, J
Anti-Ro autoantibodies, found in sera of patients with systemic lupus erythematosus, Sjogren''s syndrome, and related diseases, target the Ro ribonucleoprotein particles (RNPs). Although the polypeptide and RNA components of the Ro RNPs have been characterized, much less is know about the native structure of these particles. We have now characterized by biochemical techniques intact Ro ribonucleoprotein particles from cultured HeLa cells. These particles segregated in three discrete subpopulations with characteristic physicochemical properties: one containing hY5 RNA (RohY5 particles), one containing only hY4 RNA (RohY4 particles) and one with Hy1, hY3 and hY4 RNAs (RohY1-hY4 particles.). The RohY5 particles were purified free of contaminating ribonucleoproteins; both and La and the 60-kD Ro polypeptides were stable components of this portion of the Ro RNPs. The La RNPs co-purified with the RohY4 particles and contaminated the RohY1-hY4 RNPs. The stable association between the La and the 60 kD Ro polypeptides provides a potential macromolecular target for the linked set of anti-Ro and anti-La antibodies, and suggests a possible functional association of these polypeptides.
