To shake or not to shake: 13C-based evidence on anaerobic methane oxidation in paddy soil
To shake or not to shake: 13C-based evidence on anaerobic methane oxidation in paddy soil
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摇动还是不摇动：基于 13C 的稻田厌氧甲烷氧化证据
DOI：
10.1016/j.soilbio.2019.03.010
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发表时间：
2019-06
影响因子：
9.7
通讯作者：
Dorodnikov Maxim
中科院分区：
文献类型：
--
作者：
Lichao Fan;Shahbaz Muhammad;Tida Ge;Jinshui Wu;Dippold Michaela;Thiel Volker;Kuzyakov Yakov;Dorodnikov Maxim
甲烷的厌氧氧化（AOM）在海洋生态系统中生物产生的甲烷（CH4）进入大气之前将其去除，从而减轻了温室气体的排放。与海洋环境相比，令人惊讶的是，人们对AOM在陆地生态系统中的作用知之甚少。特别是，AOM如何控制水稻土的ch4收支尚未探索，部分反映了分析ch4周转的分析困难。迄今为止，研究土壤中AOM最常用的方法是将ch4注入顶空，在有/不摇浆的情况下对微生物进行体外培养。但是，抖动会引入各种误差和干扰。在这里，我们使用一种新的替代方法来测量水稻土壤中的AOM，该方法通过硅胶管将13c标记的ch4直接引入土壤浆中，而不摇晃。结果与经典方法（即，有和没有管和/或震动）获得的结果进行了比较。在所有批次中，注入13ch4后co2的13C富集清楚地证实了水稻土中AOM的存在。84 d的累积AOM值为0.16 ~ 0.24 μg C g−1，但震动比震动低33 ~ 80%。出乎意料的是，无论晃动与否，硅胶管对AOM的影响都不显著，这表明水（浆）中ch4浓度不是AOM的主要限制因素。在不摇晃的情况下，不加ch4的对照显示顶空/管中ch4浓度稳定增加，而摇瓶中ch4浓度在59天内持续较低。这表明震动抑制了甲烷的生成。摇浆条件下ch4的氧化量与ch4的产生量呈较强的线性相关（R2= 0.91），摇浆条件下ch4的氧化量与ch4的产生量呈幂增长回归关系。根据目前和已报道的AOM速率，中国水稻土每年可回收约2.0 Tg C的ch4，使AOM成为重要的陆地ch4汇。
The anaerobic oxidation of methane (AOM) removes most of the biologically produced methane (CH4) from marine ecosystems before it enters the atmosphere and thus mitigates greenhouse gas emissions. As compared to marine environments, surprisingly little is known about the role of AOM in terrestrial ecosystems. Particularly, how AOM controls the CH4budget of paddy soils is unexplored, partly reflecting analytical difficulties in analyzing CH4turnover. To date, the most commonly used method to study AOM in soils isin vitroincubation of microcosms with CH4injection into the headspace with/without shaking of slurry. Shaking, however, introduces various errors and disturbances. Here we measured AOM in rice paddy soil using a new alternative approach that introduced13C-labelled CH4directly into soil slurry via a silicone tube without shaking. The results were compared to those obtained by the classical approaches (i.e., with and without tubes and/or shaking). In all batches,13C enrichment of CO2after13CH4injection clearly confirmed the occurrence of AOM in paddy soil. The cumulative AOM during 84 days reached 0.16–0.24 μg C g−1dry soil without shaking, but it was 33–80% lower with shaking. Unexpectedly, the effect of silicone tubes on AOM was insignificant either with or without shaking, suggesting that the CH4concentration in water (slurry) was not the main limiting factor for AOM. Without shaking, the controls without CH4addition revealed a steady increase of CH4in the headspace/tube, whereas the CH4concentration in jars with shaking was constantly low during 59 days. This suggests that shaking inhibited methanogenesis. There was a strong linear correlation between the amount of CH4oxidized and CH4produced with shaking (R2= 0.91), whereas without shaking this relationship followed a power growth regression. Based on the current and reported AOM rates, rough upscale to paddy soils in China showed recycling of ca. 2.0 Tg C of CH4each year, making AOM a crucial terrestrial CH4sink.
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