Neutrophil dysfunction due to continuous mechanical shear exposure in mechanically assisted circulation in vitro.
Neutrophil dysfunction due to continuous mechanical shear exposure in mechanically assisted circulation in vitro.
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DOI：
10.1111/aor.14068
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发表时间：
2022-01
影响因子：
2.4
通讯作者：
Wu ZJ
中科院分区：
文献类型：
--
作者：
Sun W;Zhang J;Shah A;Arias K;Berk Z;Griffith BP;Wu ZJ
白细胞在人体免疫系统中起着重要作用。本研究的目的是评估体外泵辅助循环中中性粒细胞表型和功能的变化。人血液在三个循环流动回路中循环4小时，其中CentriMag血泵以4.5 L/min的流量在三个转速（2100、2800和4000 rpm）下分别针对三个压头（75 mmHg、150 mmHg和350 mmHg）运行。每小时采集一次血液样本，分析中性粒细胞活化状态（Mac-1、CD 62 L、CD 162）、中性粒细胞活性氧（ROS）产生、细胞凋亡和中性粒细胞吞噬作用。在三个循环中，激活的中性粒细胞的Mac-1表达和CD 62 L和CD 162受体的表面表达均随时间增加。在具有最高旋转速度的环中观察到最高水平的中性粒细胞活化。血小板-中性粒细胞聚集体（PNAs）在两个循环中以较低的转速进行性增加。在循环后1小时，PNA达到峰值，随后在具有最高转速的回路中下降。在循环后1小时，神经元ROS产生显著增加，随后在所有三个回路中以相似的水平和趋势下降。在所有三个循环中，凋亡中性粒细胞随着时间的推移而增加。三个袢的中性粒细胞吞噬能力在循环后1小时开始升高，随后下降。细胞凋亡和吞噬功能的改变依赖于转速。我们的研究表明，泵辅助循环诱导中性粒细胞活化，凋亡和功能障碍。这些变化与泵的运行条件和持续时间密切相关。
Leukocytes play an important role in the body’s immune system. The aim of this study was to assess alterations in neutrophil phenotype and function in pump-assisted circulation in-vitro. Human blood was circulated for four hours in three circulatory flow loops with a CentriMag blood pump operated at a flow of 4.5 L/min at three rotational speeds (2100, 2800, and 4000 rpm), against three pressure heads (75 mmHg, 150 mmHg, and 350 mmHg), respectively. Blood samples were collected hourly for analyses of neutrophil activation state (Mac-1, CD62L, CD162), neutrophil reactive oxygen species (ROS) production, apoptosis, and neutrophil phagocytosis. Activated neutrophils indicated by both Mac-1 expression and decreased surface expression of CD62L and CD162 receptors increased with time in three loops. The highest level of neutrophil activation was observed in the loop with the highest rotational speed. Platelet-neutrophil aggregates (PNAs) progressively increased in two loops with lower rotational speeds. PNAs peaked at one hour after circulation and decreased subsequently in the loop with the highest rotational speed. Neutrophil ROS production dramatically increased at one hour after circulation and decreased subsequently in all three loops with similar levels and trends. Apoptotic neutrophils increased with time in all three loops. Neutrophil phagocytosis capacity in three loops initially elevated at one hour after circulation and decreased subsequently. Apoptosis and altered phagocytosis were dependent on rotational speed. Our study revealed that the pump-assisted circulation induced neutrophil activation, apoptosis, and functional impairment. The alterations were strongly associated with pump operating condition and duration.
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影响因子：
4.6
作者：
Kimball, Pam M.;Flattery, Maureen;Kasirajan, Vigneshwar
通讯作者：
Kasirajan, Vigneshwar
影响因子：
4.8
作者：
Lee, Dooyoung;Schultz, Joanne B.;King, Michael R.
通讯作者：
King, Michael R.
影响因子：
4.4
作者：
Davenpeck, KL;Brummet, ME;Bochner, BS
通讯作者：
Bochner, BS
影响因子：
2.9
作者：
Ashbrook, Michael;Walenga, Jeanine M.;Bakhos, Mamdouh
通讯作者：
Bakhos, Mamdouh
影响因子：
3.2
作者：
Chen Z;Jena SK;Giridharan GA;Sobieski MA;Koenig SC;Slaughter MS;Griffith BP;Wu ZJ
通讯作者：
Wu ZJ