Increased levels of HIV RNA detected in samples with viral loads close to the detection limit collected in Plasma Preparation Tubes™ (PPT)
Increased levels of HIV RNA detected in samples with viral loads close to the detection limit collected in Plasma Preparation Tubes™ (PPT)
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DOI：
10.1016/j.jcv.2005.08.008
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发表时间：
2006-02-01
影响因子：
8.8
通讯作者：
Mayo, DR
中科院分区：
文献类型：
--
作者：
Griffith, BP;Mayo, DR
背景：准确、可靠的HIV RNA定量是HIV感染者管理的重要组成部分，阐明可能影响HIV RNA测量的因素至关重要，如使用真空血浆制备管(TM)(PPT)。目的：本研究的目的是确定PPT管中采集的病毒载量接近检测动态范围下限的血浆样品是否与标准EDTA管中采集的样品相比，HIV RNA水平升高。研究设计：比较PPT和标准EDTA管中收集的112对血浆样品中HIV RNA水平。结果：在病毒载量较高的样本中，PPT和EDTA试管之间不存在差异。然而，在病毒载量接近动态范围下限的样本中，与相应的EDTA血浆样本相比，在很大一部分PPT中检测到的艾滋病毒RNA水平更高。40%的血浆对在EDTA等分中没有检测到HIV RNA，但在相应的PPT等分中有低水平的HIV RNA。结论：这项前瞻性研究强调了在接近检测下限的样本中谨慎解释微小的瞬时病毒载量变化的必要性。(C)2005 Elsevier B.V.保留所有权利。
Background: The accurate and reliable quantification of HIV RNA is an essential part of the management of HIV infected individuals, and elucidation of factors that may affect HIV RNA measurements, such as the use of Vacutainer Plasma Preparation Tubes(TM) (PPT), is crucial.Objectives: The objective of this study was to determine if plasma samples with viral loads close to the lower limit of the dynamic range of the assay collected in PPT tubes had increased levels of HIV RNA as compared to samples collected in standard EDTA tubes.Study design: HIV RNA levels were compared in 112 paired plasma samples collected in PPT and standard EDTA tubes. All samples had been frozen prior to testing.Results: Discrepancies between PPT and EDTA tubes did not occur for samples with high viral loads. However, in samples with viral loads close to the lower limit of the dynamic range, levels of HIV RNA detected were higher in a large proportion of PPT as compared to the corresponding EDTA plasma samples. Forty percent of plasma pairs had no detectable HIV RNA in the EDTA aliquot, but had low levels of HIV RNA in the corresponding PPT aliquot.Conclusions: This prospective study underlines the need for cautious interpretation of small transient viral load changes in samples with values close to the detection limit. (C) 2005 Elsevier B.V. All rights reserved.