Optimization of sterilization methods for electrospun poly(ε-caprolactone) to enhance pre-osteoblast cell behaviors for guided bone regeneration
Optimization of sterilization methods for electrospun poly(ε-caprolactone) to enhance pre-osteoblast cell behaviors for guided bone regeneration
复制标题
优化电纺聚己内酯的灭菌方法以增强前成骨细胞行为以引导骨再生
DOI：
10.1177/0883911515598795
复制
发表时间：
2016-03-01
影响因子：
1.7
通讯作者：
Gu, Ning
中科院分区：
文献类型：
--
作者：
Dai, Yun;Xia, Yang;Gu, Ning
The aim of this study was to determine the optimal sterilization procedure for biodegradable polyester-based guided bone regeneration membranes. The effects of sterilization using low-temperature hydrogen peroxide gas plasma, 75% ethanol (EtOH; two soaking times), and ultraviolet radiation on the structure and biological properties of electrospun poly(ε-caprolactone) membranes were investigated. The results demonstrated that all were effective sterilization methods. The membranes were then assessed for surface structure, wettability, and in vitro cellular responses including osteogenic differentiation by seeding with pre-osteoblasts (MC3T3-E1 cells). The cells grew well on all the sterilized membranes. The low-temperature hydrogen peroxide gas plasma–sterilized membranes, which exhibited significantly improved hydrophilicity (p < 0.05), were better for cell osteogenic differentiation compared to the membranes sterilized by other methods. In addition, the cell behavior on the membranes sterilized by EtOH was superior to those sterilized by ultraviolet radiation. Finally, EtOH soaking time appeared to influence cell behavior. The results suggested that low-temperature hydrogen peroxide gas plasma treatment is the most promising method to sterilize electrospun poly(ε-caprolactone) membranes for guided bone regeneration.
