Effect of cadmium in the clam Ruditapes decussatus assessed by proteomic analysis
Effect of cadmium in the clam Ruditapes decussatus assessed by proteomic analysis
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DOI：
10.1016/j.aquatox.2009.07.014
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发表时间：
2009-10-04
影响因子：
4.5
通讯作者：
Bebianno, Maria Joao
中科院分区：
文献类型：
--
作者：
Chora, Suze;Starita-Geribaldi, Mireille;Bebianno, Maria Joao
镉是一种环境胁迫因子，由于其毒性、持久性和在生物群中的积累，广泛存在于水环境中。镉积累动力学表明，蛤仔对这种金属有很高的亲和力。蛋白质组学是评价污染物毒性效应的有效工具。本研究旨在探讨镉对哨种黄姑鱼鳃和消化腺的影响。交叉状蛋白质表达谱（PEP）在蛤组织暴露于镉（40 μ g-1），21天）进行了比较，未暴露的。镉诱导鳃和消化腺组织特异性蛋白质表达谱发生重大变化。这种组织依赖性的反应主要是由于镉积累，蛋白质抑制和/或自噬的差异。在两种处理组织中检测到蛋白质斑点的总体减少，在鳃中更高。去除污染物暴露后发生显著变化的部分斑点，用微液相色谱-串联质谱（LC-MS/MS）鉴定出9个斑点。在数据库中通过同源性搜索鉴定的蛋白质包括：3个（8倍）上调蛋白质，1个下调蛋白质，4个抑制蛋白质和1个诱导蛋白质。镉诱导参与细胞骨架结构维持（肌肉型肌动蛋白、收肌肌动蛋白和β-微管蛋白）、细胞维持（Rab GDP）和代谢（ALDH和MCAD，均通过从头测序鉴定）的蛋白质发生重大变化，这表明潜在的能量变化。这些结果为从生物化学和分子水平研究镉对罗氏沼虾鳃和消化腺的影响提供了有价值的信息。交叉状(C)2009爱思唯尔有限公司版权所有。
Cadmium, an environmental stressor due to its toxicity, persistence and accumulation in biota, is widespread in the aquatic environment. Cadmium accumulation kinetics have revealed that Ruditapes decussatus has a high affinity to this metal. Proteomics is an effective tool to evaluate the toxic effects of contaminants. The aim of this study was to investigate the Cd effects in the gill and digestive gland of the sentinel species R. decussatus. Protein expression profiles (PEPs) in the clam tissues exposed to Cd (40 mu g l(-1), 21 days) were compared to unexposed ones. Cd induces major changes in tissue-specific protein expression profiles in gill and digestive gland. This tissue dependent response results mainly from differences in Cd accumulation, protein inhibition and/or autophagy. An overall decrease of protein spots was detected in both treated tissues, being higher in gill. Some of the spots more drastically altered after pollutants exposure were excised and ninewere identified by micro liquid chromatography tandem mass spectrometry (LC-MS/MS). Proteins identified by homology search in databases included: three proteins (8-fold) up-regulated, one down-regulated, four suppressed and one induced. Cd induces major changes in proteins involved in cytoskeletal structure maintenance (muscle-type actin, adductor muscle actin and beta-tubulin), cell maintenance (Rab GDP) and metabolism (ALDH and MCAD, both identified by de novo sequencing) suggesting potential energetic change. They provide a valuable knowledge of Cd effects at biochemical and molecular levels in the gill and digestive gland of R. decussatus. (C) 2009 Elsevier B.V. All rights reserved.