Early stages of notochord and floor plate development in the chick embryo defined by normal and induced expression of HNF-3 beta.
Early stages of notochord and floor plate development in the chick embryo defined by normal and induced expression of HNF-3 beta.
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鸡胚脊索和底板发育的早期阶段由 HNF-3 β 的正常和诱导表达定义。
DOI：
10.1006/dbio.1995.1216
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发表时间：
1995
影响因子：
2.7
通讯作者：
Jessell,TM
中科院分区：
文献类型：
--
作者：
RuiziAltaba,A;Placzek,M;Baldassare,M;Dodd,J;Jessell,TM
我们已经克隆了编码鸡hnf-3β基因的c DNA，并使用检测hnf-3β的核糖核酸和抗体探针来监测该基因在早期胚胎中的正常和诱导表达。Hnf-3β在Koller‘s镰刀中表达，在原始条纹形成初期表达，后来在Hensen’s结节表达。在神经板和神经管阶段，hnf-3β在脊索瞬时表达，然后在底板细胞表达。在Hensen‘s结节消退之前，位于上胚层的预期底板细胞不表达hnf-3β，这提供了证据，表明通常只有当这些细胞填充在神经板的中线并覆盖在结节上时，底板的命运才被决定。体内脊索的移除阻止了底板的发育，在这种情况下，神经管腹侧中线的细胞不表达hnf-3β。脊索移植物诱导异位底板发育和hnf-3β的异位神经表达。在体外，神经板外植体被脊索细胞以接触依赖但抗环己亚胺的方式诱导表达hnf-3β，这为神经板细胞表达hnf-3β是神经板细胞对脊索诱导信号的直接反应提供了证据。
We have cloned a cDNA encoding the chick HNF-3β gene and have used RNA and antibody probes that detect HNF-3β to monitor the normal and induced expression of the gene in early embryos. HNF-3β is expressed in Koller's sickle, at the onset of primitive streak formation, and later in Hensen's node. At neural plate and neural tube stages, HNF-3 β is expressed transiently in the notochord and is then expressed by floor plate cells. Prospective floor plate cells that are located in the epiblast immediately anterior to Hensen's node prior to its regression do not express HNF-3β, providing evidence that floor plate fate is normally determined only after these cells populate the midline of the neural plate and overlie the notechord. Removal of the notochord in vivo prevents floor plate development and in this condition HNF-3β is not expressed by cells at the ventral midline of the neural tube. Notochord grafts induce ectopic floor plate development and ectopic neural expression of HNF-3 β. In vitro, neural plate explants are induced to express HNF-3β by notochord cells in a contact-dependent but cycloheximide-resistant manner, providing evidence that expression of HNF-3 β is a direct response of neural plate cells to notochord-derived inducing signals.