A novel human UDP-N-acetyl-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase, GalNAc-T7, with specificity for partial GalNAc-glycosylated acceptor substrates
A novel human UDP-N-acetyl-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase, GalNAc-T7, with specificity for partial GalNAc-glycosylated acceptor substrates
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DOI：
10.1016/s0014-5793(99)01268-5
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发表时间：
1999-10-29
期刊：
影响因子：
3.5
通讯作者：
Clausen, H
中科院分区：
文献类型：
--
作者：
Bennett, EP;Hassan, H;Clausen, H
克隆并表达了人尿苷二磷酸-N-乙酰-D-半乳糖胺：多肽N-乙酰氨基半乳糖转移酶基因家族的新成员，命名为GalNAc-T7。GalNAc-T7与该基因家族的其他特征成员相比表现出不同的性质，对部分GalNAc糖基化的受体底物表现出明显的排他性特异性。GalNAc-T7对大量非糖基化肽没有活性，但可被来自人MUC 2和大鼠颌下腺粘蛋白串联重复序列的肽底物的部分GalNAc糖基化选择性激活。GalNAc-T7的功能被认为是作为O-糖基化起始步骤中的后续酶。(C)1999年欧洲生物化学学会联合会。
A novel member of the human UDP-N-acetyl-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase gene family, designated GalNAc-T7, was cloned and expressed. GalNAc-T7 exhibited different properties compared to other characterized members of this gene family, in showing apparent exclusive specificity for partially GalNAc-glycosylated acceptor substrates. GalNAc-T7 shelved no activity with a large panel of non-glycosylated peptides, but was selectively activated by partial GalNAc glycosylation of peptide substrates derived from the tandem repeats of human MUC2 and rat submaxillary gland mucin, The function of GalNAc-T7 is suggested to be as a follow-up enzyme in the initiation step of O-glycosylation. (C) 1999 Federation of European Biochemical Societies.