Characterization of Intravacuolar Pigmented Structures in Anthocyanin-Containing Cells of Sweet Potato Suspension Cultures
Characterization of Intravacuolar Pigmented Structures in Anthocyanin-Containing Cells of Sweet Potato Suspension Cultures
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甘薯悬浮培养物含花青素细胞液泡内色素结构的表征
DOI：
10.1093/oxfordjournals.pcp.a078487
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发表时间：
1993
期刊：
影响因子：
--
通讯作者：
Hitosi Yasuda
中科院分区：
文献类型：
--
作者：
M. Nozue;H. Kubo;M. Nishimura;Akira Katou;Chiho Hattori;N. Usuda;T. Nagata;Hitosi Yasuda
研究了红薯花青素产生细胞的孔内色素结构。在连续光照下，将含2,4- d琼脂培养基培养的愈伤组织转移到不含2,4- d的液体培养基中，诱导红薯细胞形成色素结构。这些结构在液泡中发现。在60% （w/w）的蔗糖中沉淀，离心后从原生质体中分离出色素结构。电镜观察发现，这些结构既没有膜边界，也没有内部结构，在液泡中发现了强烈的亲锇性球体。在花青素积累的早期，在中央液泡中观察到许多小的亲锇球，但在细胞质中没有发现。中性红色处理后，液泡中也形成了类似的色素结构。这些观察结果表明，这些色素结构是高密度的、不溶性的、含有高浓度花青素的小球，花青素是在细胞质中合成并运送到中央液泡的。
Intravacuolar pigmented structures occurred in anthocyanin-producing cultured cells of sweet potato (Ipomoea batatas) were characterized. Formation of the pigmented structures in sweet potato cells was induced by transfer of callus cultured in 2,4-D containing agar medium into 2,4-D free liquid medium under continuous illumination. These structures were found in the vacuoles. The pigmented structures were isolated from the protoplasts by precipitation in 60% (w/w) sucrose after centrifugation. Electron microscopic observations of the anthocyanin-containing cultured cells showed these structures had neither membrane boundary nor internal structures, and were found as strongly osmiophilic globules in vacuoles. Numerous small osmiophilic globules were observed in central vacuoles at the early stage of anthocyanin accumulation, but not found in cytoplasm. Similar pigmented structures in vacuoles were also formed by treatment with neutral red. These observations indicate that these pigmented structure is the high density and insoluble globules highly concentrated with anthocyanin, which was synthesized in cytoplasm and transported to the central vacuoles.