Reversal by L-arginine of a dysfunctional arginine/nitric oxide pathway in the endothelium of the genetic diabetic BB rat.
Reversal by L-arginine of a dysfunctional arginine/nitric oxide pathway in the endothelium of the genetic diabetic BB rat.
复制标题
L-精氨酸逆转遗传性糖尿病 BB 大鼠内皮细胞中功能失调的精氨酸/一氧化氮途径。
DOI：
10.1007/s001250050767
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发表时间：
1997
期刊：
影响因子：
--
通讯作者：
Roza,AM
中科院分区：
文献类型：
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作者：
Pieper,GM;Siebeneich,W;Moore-Hilton,G;Roza,AM
我们研究了体外急性补充精氨酸对雌性遗传性糖尿病易感BB大鼠主动脉环内皮依赖性舒张的影响。与非糖尿病同窝出生的大鼠相比，糖尿病BB大鼠的环对去甲肾上腺素诱导的收缩的敏感性没有改变。在预收缩环中，乙酰胆碱产生浓度依赖性舒张，糖尿病会损害这种舒张。在对照组和糖尿病组，这种舒张作用均被硝基精氨酸阻断。加入3 mmol/L精氨酸（但不含精氨酸）可增强糖尿病环的舒张作用，与不含精氨酸的对照环相似。L精氨酸对乙酰胆碱诱导的对照环舒张无影响。与此相反，硝酸甘油诱导的舒张糖尿病环无内皮细胞没有改变由精氨酸治疗。因此，在糖尿病BB大鼠的内皮中存在一氧化氮合酶对精氨酸利用的缺陷。[Diabetologia（1997）40：910-915]
We examined the effects of acute supplementation with arginine in vitro on endothelium-dependent relaxation in aortic rings taken from female genetic, diabetes-prone BB rats. Sensitivity to norepinephrine-induced contraction was unaltered in rings of diabetic BB rats compared to rings from non-diabetic littermates. In precontracted rings, acetylcholine produced a concentration-dependent relaxation which was impaired by diabetes. This relaxation was blocked byl-nitroarginine in both control and diabetic rings. Addition of 3 mmol/ll-arginine (but notd-arginine) enhanced relaxation in diabetic rings similar to that seen in control rings without arginine.l-arginine had no effect on acetylcholine-induced relaxation in control rings. In contrast, relaxation-induced by nitroglycerin in diabetic rings without endothelium was not altered byl-arginine treatment. Thus, a defect in the utilization of arginine by nitric oxide synthase exists in the endothelium of the diabetic BB rat. [Diabetologia (1997) 40: 910–915]