Chemical assays of availability to earthworms of polycyclic aromatic hydrocarbons in soil.
Chemical assays of availability to earthworms of polycyclic aromatic hydrocarbons in soil.
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DOI：
10.1016/s0045-6535(02)00046-2
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发表时间：
2002-07
期刊：
影响因子：
8.8
通讯作者：
Jixin Tang;H. Liste;M. Alexander
中科院分区：
文献类型：
--
作者：
Jixin Tang;H. Liste;M. Alexander
有机污染物的生物可利用浓度小于通过土壤的剧烈提取所确定的浓度。由于生物利用度随特定化合物、土壤和老化时间而变化，因此需要测定生物利用度。测试了三种方法：用25%四氢呋喃（THF）水溶液、95%乙醇和C18膜进行萃取。评价进行了4个多环芳烃（PAHs）的混合物添加到5个不同的土壤和芘作为唯一的PAH添加到6个土壤，和可用性的老化和新鲜添加的化合物进行了测定。利用蚯蚓的同化作用来评估生物利用度。对于用THF萃取和吸收，蒽、芘、芘和苯并（a）芘作为混合物加入的测定相关系数（R2）超过0.85。使用C18膜进行测定的R2值为0.77或更高。单独加入芘的值分别为0.710和0.823。用乙醇测定的R2值通常超过0.87，但有时会获得较低的值。我们建议，这种溶剂或固相萃取可能是有用的，在评估PAH的生物利用度。
The bioavailable concentration of an organic pollutant is less than the concentration determined by vigorous extraction of soil. Because bioavailability varies with the particular compound, soil, and aging time, an assay for bioavailability is needed. Three methods were tested: extraction with a 25% aqueous solution of tetrahydrofuran (THF), 95% ethanol, and C18 membranes. Evaluations were conducted with a mixture of four polycyclic aromatic hydrocarbons (PAHs) added to five dissimilar soils and with pyrene as sole PAH added to six soils, and the availability of aged and freshly added compounds was determined. Assimilation by earthworms (Eisenia fetida) was used to assess bioavailability. For extraction with THF and earthworm uptake, the correlation coefficients of determination (R2) for anthracene, chrysene, pyrene, and benzo(a)pyrene added as a mixture exceeded 0.85. The R2values for assays with the C18 membrane were 0.77 or higher. The values for pyrene added alone were 0.710 and 0.823, respectively. R2values for assays with ethanol often exceeded 0.87, but lower values were sometimes obtained. We suggest that such solvent or solid-phase extractions may be useful in assessing PAH bioavailability.