Effect of pH on cellulase production and morphology of Trichoderma reesei and the application in cellulosic material hydrolysis
Effect of pH on cellulase production and morphology of Trichoderma reesei and the application in cellulosic material hydrolysis
复制标题
pH对里氏木霉纤维素酶产量和形态的影响及其在纤维素材料水解中的应用
DOI：
10.1016/j.jbiotec.2013.10.003
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发表时间：
2013-12-01
影响因子：
4.1
通讯作者：
Ma, Lijuan
中科院分区：
文献类型：
--
作者：
Li, Chen;Yang, Zhenhua;Ma, Lijuan
通过改进发酵技术实现低成本的纤维素酶仍然是纤维素生物燃料和生物化学品商业化的关键要求。pH在里氏木霉合成纤维素酶过程中起着非常重要的作用。研究了pH对纤维素酶3种组分（内切葡聚糖酶、外切葡聚糖酶、β-葡萄糖苷酶）的产酶量、产酶速率及菌丝形态的影响。为了提高纤维素酶的产量，采用分阶段pH控制策略，使纤维素酶组分的产率保持在最高水平，并使菌丝形态保持在最佳状态。纤维素酶滤纸酶活和β-葡萄糖苷酶产量分别提高了17.6%和22%。以预处理后的玉米芯为底物，考察了pH控制条件下酶的水解效果。与不控制pH值相比，发酵产率显著提高（达26.2%）。这些结果为提高纤维素酶产量的途径提供了新的知识。(C)2013爱思唯尔有限公司版权所有。
A low-cost of cellulase achieved through improving fermentation technology remains a key requirement for commercialization of cellulosic biofuels and biochemicals. pH plays a very important role in the process of cellulase synthesis by Trichoderma reesei. In this work, effects of pH on the production and production rates of three cellulase components (endoglucanase, exoglucanase, beta-glucosidase) and mycelial morphology were studied. Production rates of the cellulase components were kept highest and the mycelial morphology was maintained at the optimal status by developing a phased pH control strategy in order to improve cellulase production. Cellulase production in terms of filter paper activity and beta-glucosidase production in batch fermentation increased 17.6% and 22%. Saccharification efficiency of the enzyme obtained by pH control was evaluated by hydrolyzing pretreated corn cob. Saccharification yield increased significantly (up to 26.2%) compared with that without pH control. These results add new knowledge on approach for improving cellulase production. (C) 2013 Elsevier B.V. All rights reserved.