PO IS AN EARLY MARKER OF THE SCHWANN-CELL LINEAGE IN CHICKENS
PO IS AN EARLY MARKER OF THE SCHWANN-CELL LINEAGE IN CHICKENS
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DOI：
10.1016/0896-6273(91)90285-8
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发表时间：
1991-11-01
期刊：
影响因子：
16.2
通讯作者：
BRACKENBURY, R
中科院分区：
文献类型：
--
作者：
BHATTACHARYYA, A;FRANK, E;BRACKENBURY, R
我们已经产生了一种单克隆抗体，称为1E8，它对成熟鸡的髓鞘化和非髓鞘化雪旺细胞具有特异性。1E8首先染色神经嵴边缘的细胞；随后，位于神经管和体体之间以及硬核组中的细胞免疫阳性。HNK-1的双重标记表明，这些1e8阳性细胞代表了腹侧迁移通路中神经嵴细胞的一个子集。1e8阳性细胞后来与背根和腹根以及延伸的神经干相关。在Western blots中，1E8与与P0结合的蛋白发生反应。免疫消耗实验证实所有P0分子都携带1E8决定因子。由1E8免疫反应性确定的P0的发育分布不同于在哺乳动物中报道的P0，这表明，在鸡中，P0是雪旺细胞谱系的早期标记。
We have generated a monoclonal antibody, termed 1E8, that is specific for myelinating and nonmyelinating Schwann cells in mature chickens. 1E8 first stains cells at the edge of the neural crest; later, cells located between the neural tube and somites and in the sclerotome are immunopositive. Double labeling with HNK-1 indicates that these 1E8-positive cells represent a subset of neural crest cells in the ventral migratory pathways. 1E8-positive cells are later associated with the dorsal and ventral roots and with extending nerve trunks. In Western blots, 1E8 reacts with proteins comigrating with P0. Immunodepletion experiments establish that all P0 molecules carry the 1E8 determinant. The developmental distribution of P0, as determined by 1E8 immunoreactivity, differs from that reported for P0 in mammals and suggests that, in chicken, P0 is an early marker for the Schwann cell lineage.