Autocrine stimulation of interleukin 1 beta in acute myelogenous leukemia cells.
Autocrine stimulation of interleukin 1 beta in acute myelogenous leukemia cells.
复制标题
急性粒细胞性白血病细胞中白介素1β的自分泌刺激。
DOI：
10.1084/jem.166.5.1597
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发表时间：
1987-11-01
影响因子：
15.3
通讯作者：
Takatsuki, K
中科院分区：
文献类型：
--
作者：
Sakai, K;Hattori, T;Matsuoka, M;Asou, N;Yamamoto, S;Sagawa, K;Takatsuki, K
在 M7 急性髓性白血病患者的细胞中观察到 IL-1 导致 CD25 抗原阳性细胞显着增加。 M7白血病细胞的基础增殖和CD25抗原的表达通过添加抗IL-1β抗体以剂量依赖性方式被抑制，但不被兔抗IL-1α抗体抑制。这些白血病细胞的培养上清液含有IL-1活性，通过添加抗IL-1β抗体可以特异性抑制IL-1活性，并且Northern印迹分析检测到细胞内IL-1βmRNA。这些结果表明IL-1β的自分泌分泌参与了一些骨髓性白血病细胞的增殖。
A significant increase in CD25 antigen-positive cells by IL-1 was observed in cells of a patient with M7 acute myelogenous leukemia. Basal proliferation and expression of CD25 antigen by the M7 leukemic cells were inhibited by addition of anti-IL-1 beta antibody in a dose- dependent manner, but not by rabbit anti-IL-1 alpha antibody. Culture supernatants of these leukemic cells contained IL-1 activity, which was specifically inhibited by addition of anti-IL-1 beta antibody, and Northern blot analysis detected intracellular IL-1 beta mRNA. These results indicated that autocrine secretion of IL-1 beta was involved in proliferation of some myelogenous leukemic cells.