High-titer and productivity of L-(+)-lactic acid using exponential fed-batch fermentation with Bacillus coagulans arr4, a new thermotolerant bacterial strain
High-titer and productivity of L-(+)-lactic acid using exponential fed-batch fermentation with Bacillus coagulans arr4, a new thermotolerant bacterial strain
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DOI：
10.1007/s13205-018-1232-0
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发表时间：
2018-04-07
期刊：
影响因子：
2.8
通讯作者：
Contiero, Jonas
中科院分区：
文献类型：
--
作者：
Coelho, Luciana Fontes;Beitel, Susan Michelz;Contiero, Jonas
凝结芽孢杆菌arr 4是一株耐热微生物，具有利用砂糖和酵母膏生产L-（+）-乳酸的巨大潜力。Ca（OH）（2）的L-（+）-乳酸产量最高。在50 ℃、pH6.5、初始砂糖浓度为100 g/L、使用砂糖溶液（900 g/L）和酵母提取物（1%）的指数流加中，在39 h时观察到L-（+）-乳酸的最大产量（206.81 g/L）。5.3 g/L h，产率97%，无糖残留。与指数流加发酵相比，采用白砂糖和酵母膏进行流加发酵，L（+）乳酸产量提高了133.22%，菌体干重提高了83.29%。这项研究提出了在文献中观察到的最高乳酸生产率，对耐热芽孢杆菌的发酵以及创新和高效的纯化技术，使用低成本的物质，如硅藻土和木炭。乳酸的回收率为86%，蛋白质去除率为100%，发酵培养基（棕色）变为无色溶液。
Bacillus coagulans arr4 is a thermotolerant microorganism with great biotechnological potential for L-(+)-lactic acid production from granulated sugar and yeast extract. The highest L-(+)-lactic acid production was obtained with Ca(OH)(2). The maximum production of L-(+)-lactic acid (206.81 g/L) was observed in exponential feeding using granulated sugar solution (900 g/L) and yeast extract (1%) at 50 degrees C, pH 6.5, and initial granulated sugar concentration of 100 g/L at 39 h. 5.3 g/L h productivity and 97% yield were observed, and no sugar remained. Comparing the simple batch with exponential fed-batch fermentation, the L(+) lactic acid production was improved in 133.22% and dry cell weight was improved in 83.29%, using granulated sugar and yeast extract. This study presents the highest productivity of lactic acid ever observed in the literature, on the fermentation of thermotolerant Bacillus sp. as well as an innovative and high-efficiency purification technology, using low-cost substances as Celite and charcoal. The recovery of lactic acid was 86%, with 100% protein removal, and the fermentation medium (brown color) became a colorless solution.