Selective solid-phase extraction of cholesterol using molecularly imprinted polymers and its application in different biological samples.
Selective solid-phase extraction of cholesterol using molecularly imprinted polymers and its application in different biological samples.
复制标题
DOI：
10.1016/j.jpba.2006.05.022
复制
发表时间：
2006-11
影响因子：
3.4
通讯作者：
Yun Shi;Jiang-hua Zhang;Dan Shi;M. Jiang;Ye-xiang Zhu;Surong Mei;Yikai Zhou;K. Dai;Bin Lu
中科院分区：
文献类型：
--
作者：
Yun Shi;Jiang-hua Zhang;Dan Shi;M. Jiang;Ye-xiang Zhu;Surong Mei;Yikai Zhou;K. Dai;Bin Lu
采用紫外引发聚合法制备了胆固醇非共价分子印迹聚合物（MIPs）。具有最高的结合选择性和能力的聚合物被用作固相萃取（SPE）吸附剂，用于从不同的生物样品（人血清，牛奶，蛋黄，虾，猪肉和牛肉）中直接提取胆固醇。优化了分子印迹固相萃取柱（MISPE）的提取条件，最佳方案为：正己烷预处理，上样，正己烷洗涤，正己烷：甲苯=9：1洗涤，氯仿：乙醇：冰醋酸=3：1：1洗脱。胆固醇MISPE选择性地识别、有效地捕获和预浓缩胆固醇，浓度范围为10-80μg/mL。回收率范围为80.6%-92.7%，R.S.D.低于9.8%。在最佳条件下，人血清、蛋黄、牛奶、虾、猪肉和牛肉的MISPE加标回收率分别为91.1%、80.4%、86.6%、78.2%、81.4%和80.1%。与C18固相萃取相比，MISPE几乎去除了所有的基质干扰，获得了更好的基线和更高的选择性。
Non-covalent molecularly imprinted polymers (MIPs) of cholesterol were prepared by UV initiated polymerization. A polymer that had the highest binding selectivity and capability was used as solid-phase extraction (SPE) sorbents for direct extraction of cholesterol from different biological samples (human serum, cow milk, yolk, shrimp, pork and beef). The extraction conditions of molecularly imprinted SPE (MISPE) were optimized and the optimum protocol was: conditioning MISPE cartridges with n-hexane, loading with n-hexane, washing with n-hexane and n-hexane:toluene=9:1, respectively, then eluting with chloroform:ethanol:acetic acid=3:1:1. Cholesterol MISPE selectively recognized, effectively trapped and pre-concentrated cholesterol over a concentration range of 10–80μg/mL. Recoveries ranged from 80.6% to 92.7%, with R.S.D. lower than 9.8%. Under the optimal condition, MISPE recoveries of spiked human serum, yolk, cow milk, shrimp, pork and beef were 91.1%, 80.4%, 86.6%, 78.2%, 81.4% and 80.1%, respectively. Compared with C18 SPE, almost all of the matrix interferences were removed after MISPE, and better baselines and higher selectivity were achieved.