Superoxide dismutase mimetic nanoceria restrains cerulein induced acute pancreatitis
Superoxide dismutase mimetic nanoceria restrains cerulein induced acute pancreatitis
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DOI：
10.2217/nnm-2018-0318
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发表时间：
2019-07-01
期刊：
影响因子：
5.5
通讯作者：
Godugu, Chandraiah
中科院分区：
文献类型：
--
作者：
Khurana, Amit;Anchi, Pratibha;Godugu, Chandraiah
目的：研究纳米氧化铈（NC）对蛙皮素致胰腺炎的影响。方法：对NC进行表征，并在小鼠巨噬细胞中进行体外研究。对蛙皮素诱导的胰腺炎进行体内效应测试。结果如下：在体外用NC处理显著地保护巨噬细胞免受脂多糖诱导的炎症和氧化应激，如从2 '，7'-二氯荧光素二乙酸酯、JC-1和MitoSox染色的结果所证明的。在体内治疗与NC表现出强大的超氧化物歧化酶和过氧化氢酶模拟活性，抗胰腺炎活性和改善组织学。此外，它降低了p65-NF-κ B的表达和组蛋白H3在赖氨酸K14、K56和K79残基处的乙酰化。结论：我们首次证明NC可能是治疗胰腺炎的一种有希望的候选药物。
Aim: The present study was carried out to assess the effect of nanoceria (NC) on pancreatic inflammation caused by cerulein. Methods: NC was characterized and in vitro studies were carried out in murine macrophages. The in vivo effects were tested on cerulein-induced pancreatitis. Results: In vitro treatment with NC remarkably protected macrophages from lipopolysaccharide-induced inflammation and oxidative stress as evident from the results of 2',7'-dichlorofluorescin diacetate, JC-1 and MitoSox staining. In vivo treatment with NC showed potent superoxide dismutase and catalase mimetic activity, antipancreatitis activity and improved histology. Furthermore, it reduced the expression of p65-NF-kappa B and acetylation of histone H3 at lysine K14, K56 and K79 residues. Conclusion: We for the first time, demonstrate that NC may be a promising candidate for the therapy of pancreatitis.