Trichuris vulpis and T. trichiura infections among schoolchildren of a rural community in northwestern Thailand: the possible role of dogs in disease transmission
Trichuris vulpis and T. trichiura infections among schoolchildren of a rural community in northwestern Thailand: the possible role of dogs in disease transmission
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DOI：
10.2478/abm-2010-0006
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发表时间：
2010-02-01
影响因子：
0.6
通讯作者：
Jongwutiwes, Somchai
中科院分区：
文献类型：
--
作者：
Areekul, Pannatat;Putaporntip, Chaturong;Jongwutiwes, Somchai
背景：鞭虫病是由鞭虫引起的一种重要的土源性蠕虫感染。世界上大约十分之一的人口可能被感染。顺便说一句，T。据报道，狐狸或狗鞭虫根据卵的大小感染人类。目的：建立一种区分鞭虫和鞭虫卵的PCR方法，并调查泰国农村地区鞭虫在人和犬中的流行情况。材料和方法：我们确定和比较小亚基核糖体RNA序列的两种鞭虫发展种特异性PCR诊断。经过验证的方法，我们进行了横截面调查，在塔松扬区在德省，泰国西北部在2008年。结果：共采集粪便标本五十六份，粪便中检出鞭虫卵。PCR检测结果显示50例为T。毛尾丝虫和毛尾丝虫共感染6例（10.7%）。trichiura和T.狐狸虽然鞭虫卵的大小为种的鉴别提供了一定的线索，但鞭虫卵的长度在不同样品间存在显著差异，可能导致误诊。在该社区周围游荡的狗的粪便样本中检测到狐狸卵，突出了狗在该人群中传播人和狗鞭虫的潜在储存者作用。
Background: Trichuriasis is an important soil-transmitted helminth infection caused by Trichuris trichiura. About one-tenth of the world population may be infected. Incidentally, T. vulpis or dog whipworm has been reported to infect humans based on the egg size. However, an overlapping egg dimension occurs between T trichiura and T vulpis leading to the potential for misdiagnosis.Objective: Develope a PCR method to differentiate T trichiura and T vulpis eggs in stool samples and to investigate the prevalence of both whipworms in humans and dogs in a rural community in Thailand.Materials and methods: We determined and compared the small subunit ribosomal RNA sequences of both species of whipworms for developing species-specific PCR diagnosis. After validation of the method, we conducted a cross-sectional survey at Ta Song Yang District in Tak Province, northwestern Thailand in 2008. Stool samples were randomly recruited from 80 schoolchildren (36 males, 44 females) and 79 dogs in this community.Results: Fifty-six individuals harbored Trichuris eggs in their stools. The PCR-based diagnosis revealed that 50 cases were infected with T. trichiura and six (10.7%) were co-infected with both T. trichiura and T. vulpis. Although the dimension of Trichuris eggs provided some diagnostic clues for species differentiation, a remarkable variation in the length of these whipworm eggs was observed among samples that could lead to misdiagnosis.Conclusion: Both T.t richiura and T. vulpis eggs were detected in stool samples of dogs that roamed around this community, highlighting the potential reservoir role of dogs in the transmission of both human and dog whipworms in this population.