Progesterone does not affect the amount of mRNA for gonadotropins in the anterior pituitary gland of ovariectomized ewes.
Progesterone does not affect the amount of mRNA for gonadotropins in the anterior pituitary gland of ovariectomized ewes.
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黄体酮不影响卵巢切除母羊垂体前叶中促性腺激素 mRNA 的量。
DOI：
10.1095/biolreprod37.5.1225
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发表时间：
1987
影响因子：
3.6
通讯作者：
Nett,TM
中科院分区：
文献类型：
--
作者：
Hamernik,DL;Kim,KE;Maurer,RA;Nett,TM
为了评估黄体酮对促性腺激素合成和分泌的影响，切除卵巢的母羊要么用黄体酮（n = 5）处理3周，要么在发情季节作为对照（n = 5）。治疗3周后，从接受黄体酮治疗和切除卵巢的母羊身上采集血样。采集血样后，从所有母羊身上采集下丘脑和垂体组织。每个垂体的一半用于测定黄体生成素（LH）和卵泡刺激素（FSH）的含量，以及促性腺激素释放激素（GnRH）受体的数量。在每个垂体的另一半中测量 LH β 亚基、FSH β 亚基、α 亚基、生长激素和催乳素的 mRNA 量。黄体酮治疗降低了 LH 的平均血清浓度 (p < 0.001)，但降低了 FSH (p > 0.05)。此外，孕酮降低了 LH 脉冲总数（p < 0.05）。我们无法检测到 FSH 的脉冲释放。下丘脑 GnRH 含量、GnRH 受体数量、垂体促性腺激素含量以及 LH β 亚基、FSH β 亚基、α 亚基、生长激素和催乳素的 mRNA 不受黄体酮治疗的影响 (p > 0.05)。因此，用黄体酮治疗后，LH（而非 FSH）的血清浓度会降低。然而，这种效应并不是由于 LH β 或 α 亚基的 mRNA 稳态量减少所致。
To evaluate the effect of progesterone on the synthesis and secretion of gonadotropins, ovariectomized ewes either were treated with progesterone (n=5) for 3 wk or served as controls (n=5) during the anestrous season. After treatment for 3 wk, blood samples were collected from progesterone-treated and ovariectomized ewes. After collection of blood samples, hypothalamic and hypophyseal tissues were collected from all ewes. Half of each pituitary was used to determine the content of luteinizing hormone (LH) and follicle-stimulating hormone (FSH), and the number of receptors for gonadotropin-releasing hormone (GnRH). The amounts of mRNA for LH beta subunit, FSH beta subunit, alpha subunit, growth hormone, and prolactin were measured in the other half of each pituitary. Treatment with progesterone reduced mean serum concentrations of LH (p < 0.001) but ot FSH (p > 0.05). Further, progesterone decreased (p < 0.05) the total number of pulses of LH. We were unable to detect pulsatile release of FSH. Hypothalamic content of GnRH, number of receptors for GnRH, pituitary content of gonadotropins and mRNA for LH beta subunit, FSH beta subunit, alpha subunit, growth hormone, and prolactin were not affected (p > 0.05) by treatment with progesterone. Thus, after treatment with progesterone, serum concentrations of LH (but not FSH) are decreased. This effect, however, is not due to a decrease in the steady-state amount of mRNA for LH beta or alpha subunits.