Low-intensity pulsed ultrasound accelerates nerve regeneration following inferior alveolar nerve transection in rats.
Low-intensity pulsed ultrasound accelerates nerve regeneration following inferior alveolar nerve transection in rats.
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DOI：
10.1111/eos.12271
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发表时间：
2016-04
影响因子：
1.9
通讯作者：
Mai Sato;M. Motoyoshi;M. Shinoda;K. Iwata;Noriyoshi Shimizu
中科院分区：
文献类型：
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作者：
Mai Sato;M. Motoyoshi;M. Shinoda;K. Iwata;Noriyoshi Shimizu
下肺泡神经（IAN）损伤通常由口面部手术或外伤引起，在受IAN支配的口面部区域引起感觉丧失。然而，目前还没有有效的治疗口面部感觉丧失的方法。我们通过将横断的IAN暴露于低强度脉冲超声（LIPUS）来确定是否可以恢复IAN横断后颏孔上方面部皮肤的感觉丧失。对7周龄雄性Sprague-Dawley大鼠进行下肺泡神经横断术（IANX）。在IANX后第7天，每天LIPUS（从第0天开始）对横切IAN的影响，就对颏孔以上面部皮肤机械刺激的敏感性而言，进行了检查。此外，采用逆行神经示踪技术，对每天使用LIPUS治疗IANX大鼠神经孔上方面部皮肤的三叉神经节（TG）神经元数量进行计数。每天将横断的IAN暴露在LIPUS下，可显著促进脑孔上方面部皮肤机械刺激后的头部退出阈值的恢复，并且与假手术或未暴露LIPUS的大鼠相比，每天接受LIPUS治疗的IANX大鼠神经支配颏孔上方面部皮肤的TG神经元数量显著增加。每天用LIPUS治疗横断的IAN残肢，促进了形态和功能的再生，表明LIPUS是治疗IAN损伤的一种有效的新方法。
Inferior alveolar nerve (IAN) injury, which is frequently caused by orofacial surgery or trauma, induces sensory loss in orofacial regions innervated by the IAN. However, no effective treatment for orofacial sensory loss currently exists. We determined whether sensory loss in facial skin above the mental foramen following IAN transection was recovered by exposure of the transected IAN to low-intensity pulsed ultrasound (LIPUS). Inferior alveolar nerve transection (IANX) was performed in 7-wk-old male Sprague-Dawley rats. On day 7 after IANX, the effect of daily LIPUS (from day 0) on the transected IAN, in terms of sensitivity to mechanical stimulation of the facial skin above the mental foramen, was examined. Moreover, the number of trigeminal ganglion (TG) neurons innervating the facial skin above the mental foramen of rats with IANX treated daily with LIPUS was counted using the retrograde neurotracing technique. Daily exposure of the transected IAN to LIPUS significantly promoted recovery of the head-withdrawal threshold in response to mechanical stimulation of the facial skin above the mental foramen, and the number of TG neurons innervating the facial skin above mental foramen was significantly increased in rats with IANX treated daily with LIPUS compared with sham or LIPUS-unexposed rats. Daily treatment of stumps of the transected IAN with LIPUS facilitated morphological and functional regeneration, suggesting that LIPUS is an effective and novel therapy for IAN injury.