Spider flagelliform silk: lessons in protein design, gene structure, and molecular evolution
Spider flagelliform silk: lessons in protein design, gene structure, and molecular evolution
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DOI：
10.1002/bies.1105
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发表时间：
2001-08-01
期刊：
影响因子：
4
通讯作者：
Lewis, RV
中科院分区：
文献类型：
--
作者：
Hayashi, CY;Lewis, RV
蜘蛛可以纺出多种类型的丝，这些丝以其卓越的机械性能而闻名。用于捕获球网螺旋的鞭状丝是少数具有 cDNA 和基因组 DNA 数据特征的丝之一。这种丝心蛋白由三种类型的氨基酸序列基序的重复集合组成。主要的子重复 GPGGX 可能形成 β 转角，并且这些转角的串联阵列被认为会产生 β 螺旋。这些弹簧状的螺旋对于捕捉丝的拉伸和反冲的卓越能力可能至关重要。发现每个基序集合对应于鞭毛状基因内的不同外显子。外显子之间的序列相似性模式表明基因内协同进化。令人惊讶的是，重复的外显子之间的内含子也彼此同质化。鞭状丝基因中这种不寻常的分子结构对蜘蛛丝蛋白的进化和维持具有重要意义。 (C) 2001 约翰威利父子公司
Spiders spin multiple types of silks that are renowned for their superb mechanical properties. Flagelliform silk, used in the capture spiral of an orb-web, is one of the few silks characterized by both cDNA and genomic DNA data. This fibroin is composed of repeating ensembles of three types of amino acid sequence motifs. The predominant subrepeat, GPGGX, likely forms a beta -turn, and tandem arrays of these turns are thought to create beta -spirals. These spring-like helices may be critical for the exceptional ability of capture silk to stretch and recoil. Each ensemble of motifs was found to correspond to a different exon within the flagelliform gene. The pattern of sequence similarity among exons indicates intragenic concerted evolution. Surprisingly, the introns between the iterated exons are also homogenized with each other. This unusual molecular architecture in the flagelliform silk gene has implications for the evolution and maintenance of spider silk proteins. (C) 2001 John Wiley & Sons, Inc.