SPOROTRICHUM-THERMOPHILE GROWTH, CELLULOSE DEGRADATION, AND CELLULASE ACTIVITY
SPOROTRICHUM-THERMOPHILE GROWTH, CELLULOSE DEGRADATION, AND CELLULASE ACTIVITY
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DOI：
10.1128/aem.53.9.2175-2182.1987
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发表时间：
1987-09-01
影响因子：
4.4
通讯作者：
MAHESHWARI, R
中科院分区：
文献类型：
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作者：
BHAT, KM;MAHESHWARI, R
嗜热真菌Sporotrichum thermophile的胞外纤维素酶系统的组分的活性在菌株之间显示出明显的差异;葡糖苷酶（EC 3.2.1.21）是最可变的组分。虽然其内切葡聚糖酶（EC 3.2.1.4.）和外切葡聚糖酶（EC 3.2.1.91）活性显著降低，S.嗜热木霉降解纤维素酶的速度比里氏木霉快。β的产生-葡萄糖苷酶的活性滞后于内切葡聚糖酶和外切葡聚糖酶。后者的活动是在积极的增长。当生长被抑制放线菌酮处理，纤维素的水解是低于对照，尽管存在的内切葡聚糖酶和外切葡聚糖酶的活动在培养基中。纤维素的降解是一个与生长相关的过程，纤维素酶制剂仅在有限程度上水解纤维素。生长速度和细胞密度的S。嗜热菌在含有纤维素或葡萄糖的培养基中相似。真菌在含有纤维素或乳糖（纤维素酶活性的诱导剂）的培养基中发育的一个显著特征是繁殖单位的快速分化和菌丝细胞的自溶，以释放能够立即恢复生长的繁殖体。
The activity of components of the extracellular cellulase system of the thermophilic fungus Sporotrichum thermophile showed appreciable differences between strains; .beta.-glucosidase (EC 3.2.1.21) was the most variable component. Although its endoglucanase (EC 3.2.1.4.) and exoglucanase (EC 3.2.1.91) activities were markedly lower, S. thermophile degraded cellulase faster than Trichoderma reesei. The production of .beta.-glucosidase lagged behind that of endoglucanase and exoglucanase. The latter activities were produced during active growth. When growth was inhibited by cycloheximide treatment, the hydrolysis of cellulose was lower than in the control in spite of the presence of both endoglucanase and exoglucanase activities in the culture medium. Degradation of cellulose was a growth-associated process, with cellulase preparations hydrolyzing cellulose only to a limited extent. The growth rate and cell density of S. thermophile were similar in media containing cellulose or glucose. A distinctive feature of fungal development in media incorporating cellulose or lactose (inducers of cellulase activity) was the rapid differentiation of reproductive units and autolysis of hyphal cells to liberate propagules which were capable of renewing growth immediately.