Caspase-3/7 inhibition alters cell morphology in mitomycin-C treated chondrocytes.
Caspase-3/7 inhibition alters cell morphology in mitomycin-C treated chondrocytes.
复制标题
Caspase-3/7 抑制会改变丝裂霉素 C 处理的软骨细胞的细胞形态。
DOI：
10.1002/jcp.20373
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发表时间：
2005
期刊：
影响因子：
--
通讯作者：
Lust,George
中科院分区：
文献类型：
--
作者：
Clements,KristenM;Burton-Wurster,Nancy;Nuttall,MarkE;Lust,George
Apoptosis may play a role in osteoarthritis (OA). Apoptosis can proceed via two different pathways depending on the stimulus. However, both pathways converge upon the effector caspases, caspases‐3 and ‐7. In some systems inhibition of caspases‐3 and ‐7 can prevent apoptosis and may therefore have important therapeutic implications. To confirm this, apoptosis was induced in canine chondrocytes with mitomycin‐c (MMC), either in the presence or absence of the general caspase inhibitor, Z‐VAD FMK, or a specific caspase‐3/7 inhibitor. Z‐VAD FMK prevented MMC induced cell death. In contrast, inhibition of caspases‐3 and ‐7 in the presence of MMC induced morphological changes that could be described as necrotic‐like or paraptotic‐like but did not prevent cell death. The addition of an inhibitor of caspase‐8 or caspase‐9 along with inhibitor of caspase‐3/7 was required to reduce cell death. The morphological changes did not occur in the presence of the caspase‐3/7 inhibitor alone and could be prevented by addition of Z‐VAD FMK. These data lead to the conclusion that, if the apoptotic program cannot be completed, the cells are pushed into a necrotic or other nonapoptotic mode of death which may involve caspase‐8 and/or caspase‐9. © 2005 Wiley‐Liss, Inc.
