GWAS on the Attack by Aspen Borer Saperda calcarata on Black Cottonwood Trees Reveals a Response Mechanism Involving Secondary Metabolism and Independence of Tree Architecture
GWAS on the Attack by Aspen Borer Saperda calcarata on Black Cottonwood Trees Reveals a Response Mechanism Involving Secondary Metabolism and Independence of Tree Architecture
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DOI：
10.3390/f14061129
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发表时间：
2023-05
期刊：
影响因子：
2.9
通讯作者：
S. Sepúlveda;D. Neale;J. Holliday;Randi A. Famula;O. Fiehn;B. Stanton;Fernando P. Guerra
中科院分区：
文献类型：
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作者：
S. Sepúlveda;D. Neale;J. Holliday;Randi A. Famula;O. Fiehn;B. Stanton;Fernando P. Guerra
黑棉白杨（Populus crucarpa）是一种重要的经济树种，也是一种优秀的研究模式。白杨螟（Saperda calcarata）对北美的杨树和其他河岸物种造成不可逆转的损害。这种昆虫可以产生多种影响，从茎中的一些画廊到树木死亡。尽管这种树-虫相互作用在生态和商业上具有重要意义，但是，梨对S. calcarata很少被理解。在这项研究中，一个共同的花园试验，在戴维斯，加州，建立了大果梨种源，在第二年的增长，关于S。是自然爆发的。使用629 k外显子SNP进行全基因组关联研究（GWAS）以评估基因组变异与昆虫攻击之间的关系。树的结构，在每株植物的茎数方面，木材代谢组也包括在内。昆虫的攻击是独立的每棵树的茎数。所进行的GWAS鉴定了属于相同数量的基因模型的三个显著相关的SNP标记（q值< 0.2），编码参与信号转导机制和次级代谢产物产生的蛋白质，包括R-扁桃腈裂解酶、染色体结构域-解旋酶-DNA结合家族蛋白和富含亮氨酸的重复蛋白。这些结果与植物和树木防御途径的现有知识相一致，有助于扩大对黑棉白杨对木材蛀虫的防御反应机制的理解。
Black cottonwood (Populus trichocarpa) is a species of economic interest and an outstanding study model. The aspen borer (Saperda calcarata) causes irreversible damage to poplars and other riparian species in North America. The insect can produce multiple effects ranging from the presence of some galleries in the stem to tree death. Despite the ecological and commercial importance of this tree–insect interaction, the genetic mechanisms underlying the response of P. trichocarpa to S. calcarata are scarcely understood. In this study, a common garden trial of P. trichocarpa provenances, established in Davis, California, was assessed at the second year of growth, regarding the infestation of S. calcarata from a natural outbreak. A genome-wide association study (GWAS) was conducted using 629k of exonic SNPs to assess the relationship between genomic variation and insect attack. Tree architecture, in terms of stem number per plant, and the wood metabolome were also included. Insect attack was independent of the number of stems per tree. The performed GWAS identified three significantly associated SNP markers (q-value < 0.2) belonging to the same number of gene models, encoding proteins involved in signal transduction mechanisms and secondary metabolite production, including that of R-mandelonitrile lyase, Chromodomain-helicase-DNA-binding family protein, and Leucine-rich repeat protein. These results are aligned with the current knowledge of defensive pathways in plants and trees, helping to expand the understanding of the defensive response mechanisms of black cottonwood against wood borer insects.