Nucleases from Prevotella intermedia can degrade neutrophil extracellular traps.
Nucleases from Prevotella intermedia can degrade neutrophil extracellular traps.
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DOI：
10.1111/omi.12171
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发表时间：
2017-08
影响因子：
3.7
通讯作者：
Kuwata H
中科院分区：
文献类型：
--
作者：
Doke M;Fukamachi H;Morisaki H;Arimoto T;Kataoka H;Kuwata H
牙周炎是由龈下菌斑中的牙周细菌引起的炎症性疾病。这些细菌能够通过逃避宿主免疫反应而在牙周区域定殖。中性粒细胞是宿主抵御感染的第一道防线，它使用各种策略来杀死入侵的病原体，包括中性粒细胞胞外陷阱（NET）。这些是细胞外网状纤维，包含 DNA 和抗菌成分，如组蛋白、LL-37、防御素、髓过氧化物酶和来自中性粒细胞的中性粒细胞弹性蛋白酶，可解除和杀死细胞外细菌。细菌核酸酶降解 NET 以逃避 NET 杀死。现在已经表明，细胞外核酸酶使细菌能够逃避这种宿主抗菌机制，导致致病性增加。在这里，我们比较了主要革兰氏阴性牙周病原菌、牙龈卟啉单胞菌、中间普氏菌、具核梭杆菌和放线菌聚集菌的 DNA 降解活性。我们发现 Pr。 中间体表现出最高的 DNA 降解活性。 Pr 的基因组搜索。 intermedia揭示了两个基因的存在，nucA和nucD，推测编码分泌性核酸酶，尽管它们的酶活性和生物活性尚不清楚。我们从 Pr 中克隆了 nucA 和 nucD 编码核酸酶。 intermedia ATCC 25611 并表征了它们的基因产物。重组 NucA 和 NucD 消化 DNA 和 RNA，需要 Mg2+ 和 Ca2+ 才能获得最佳活性。此外，NucA 和 NucD 能够降解包含 NET 的 DNA 基质。
Periodontitis is an inflammatory disease caused by periodontal bacteria in subgingival plaque. These bacteria are able to colonize the periodontal region by evading the host immune response. Neutrophils, the host's first line of defense against infection, use various strategies to kill invading pathogens, including neutrophil extracellular traps (NETs). These are extracellular net‐like fibers comprising DNA and antimicrobial components such as histones, LL‐37, defensins, myeloperoxidase, and neutrophil elastase from neutrophils that disarm and kill bacteria extracellularly. Bacterial nuclease degrades the NETs to escape NET killing. It has now been shown that extracellular nucleases enable bacteria to evade this host antimicrobial mechanism, leading to increased pathogenicity. Here, we compared the DNA degradation activity of major Gram‐negative periodontopathogenic bacteria, Porphyromonas gingivalis, Prevotella intermedia, Fusobacterium nucleatum, and Aggregatibacter actinomycetemcomitans. We found that Pr. intermedia showed the highest DNA degradation activity. A genome search of Pr. intermedia revealed the presence of two genes, nucA and nucD, putatively encoding secreted nucleases, although their enzymatic and biological activities are unknown. We cloned nucA‐ and nucD‐encoding nucleases from Pr. intermedia ATCC 25611 and characterized their gene products. Recombinant NucA and NucD digested DNA and RNA, which required both Mg2+ and Ca2+ for optimal activity. In addition, NucA and NucD were able to degrade the DNA matrix comprising NETs.
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影响因子：
7.6
作者：
White, P. C.;Chicca, I. J.;Chapple, I. L. C.
通讯作者：
Chapple, I. L. C.
DOI：
10.1084/jem.20100239
发表时间：
2010-08-30
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Li P;Li M;Lindberg MR;Kennett MJ;Xiong N;Wang Y
通讯作者：
Wang Y
影响因子：
2.8
作者：
de Buhr, Nicole;Neumann, Ariane;Baums, Christoph G.
通讯作者：
Baums, Christoph G.
影响因子：
14.9
作者：
FRIEDHOFF, P;GIMADUTDINOW, O;PINGOUD, A
通讯作者：
PINGOUD, A
影响因子：
5.6
作者：
Jones, SJ;Worrall, AF;Connolly, BA
通讯作者：
Connolly, BA