Purification of virus-like particles from male-sterile vicia faba and detection by elisa in crude leaf extracts
Purification of virus-like particles from male-sterile vicia faba and detection by elisa in crude leaf extracts
复制标题
DOI：
10.1016/0168-9452(88)90178-1
复制
发表时间：
1988
期刊：
影响因子：
5.2
通讯作者：
P. Dulieu;F. Penin;D. Gautheron
中科院分区：
文献类型：
--
作者：
P. Dulieu;F. Penin;D. Gautheron
A method purifying virus-like particles (VLPs) to physical homogeneity from male-sterileVicia faba(“447” cytoplasm, Ad 23 line) is described for the first time. Purified VLPs were used to prepare a specific antiserum. An Enzyme Linked Immunosorbant Assay (ELISA) was developed with antibodies purified from the specific antiserum in order to detect the presence of VLP in very small amounts of crude leaf extracts (approx. 20mg of fresh tissues). This ELISA allows the detection of male-sterileVicia fabaindividuals at a very early decvelopmental stage.
