Role of Hairpin-Quadruplex DNA Secondary Structural Conversion in the Promoter of hnRNP K in Gene Transcriptional Regulation
Role of Hairpin-Quadruplex DNA Secondary Structural Conversion in the Promoter of hnRNP K in Gene Transcriptional Regulation
复制标题
发夹四链体 DNA 二级结构转换在 hnRNP K 启动子中在基因转录调控中的作用
DOI：
10.1021/acs.orglett.5b02310
复制
发表时间：
2015
期刊：
影响因子：
5.2
通讯作者：
Li Ding
中科院分区：
文献类型：
--
作者：
Qiu Jun;Liu Jinggong;Chen Shuobin;Ou Tian-Miao;Tan Jia-Heng;Gu Lian-Quan;Huang Zhi-Shu;Li Ding
hnRNP K癌基因的启动子被发现在同一条DNA链上含有富含G/C的序列，该序列可以形成可相互转换的G-四链体、i-基序和发夹结构。 CNBP蛋白可以结合并稳定G-四链体，诱导发夹转化为G-四链体，导致hnRNP K转录下调。相反，皮质酮可以结合并稳定发夹，诱导 G-四链体转化为发夹，导致 hnRNP K 基因转录上调。
The promoter of hnRNP K oncogene was found to contain a G/C-rich sequence on the same DNA strand, which can form interconvertible G-quadruplex, i-motif, and hairpin structures. Protein CNBP could bind and stabilize the G-quadruplex, inducing transformation of the hairpin into the G-quadruplex, resulting in down-regulation of hnRNP K transcription. In contrast, Corticosterone could bind and stabilize the hairpin, inducing transformation of the G-quadruplex into the hairpin, resulting in up-regulation of hnRNP K gene transcription.