Pathogenicity of entomopathogenic fungi to eggs and larvae of Spodoptera litura, the common cutworm
Pathogenicity of entomopathogenic fungi to eggs and larvae of Spodoptera litura, the common cutworm
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DOI：
10.1080/09583150903205069
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发表时间：
2009-01-01
影响因子：
1.4
通讯作者：
Tiwary, Bhupendra N.
中科院分区：
文献类型：
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作者：
Anand, Rajesh;Tiwary, Bhupendra N.
采用不同浓度的绿僵菌(Metarhizium anisopliae var. anisopliae (Metschnikov) Sorokin （ARSEF 7487）、白僵菌(Lecanicillium muscarium (Petch) Zare W Gams （ARSEF 7037和ARSEF 6118）、红虫草（Cordyceps cardinalis） （ARSEF 7193）、红土镰刀菌Nees （ARSEF 8291/MTCC 9050）和曲霉（Aspergillus sp. ARSEF 8519） 6株真菌分生菌悬浮液处理斜纹夜蛾卵和2龄幼虫。在无鳞虫卵的生物测定中，绿僵菌、红僵菌、红土赤霉病菌和曲霉在106孢子/mL以上死亡率为100%。然而，在有鳞片的卵块中，白僵菌（ARSEF 6118）的孵化率最高（56%），而绿僵菌的孵化率最低，其次是白僵菌（ARSEF 7037）、曲霉（Aspergillus sp.）、红土菌（F. lateritium）和红僵菌（C. cardinalis）。还发现幼虫对所有分离物均有剂量依赖性。3个有潜力的分离株（ARSEF 7037）对绿僵菌、红土菌和白僵菌的平均死亡率分别为88%、89%和77%，约为108个分生孢子/mL。当幼虫和叶片（作为食物提供）用大约108个分生孢子/mL处理时，这些分离株的死亡率进一步增加。基于致死率和LC50值，我们认为绿僵菌M. anisopliae、红土菌F. lateritium和白僵菌L. muscarium （ARSEF 7037）可作为水稻切虫的潜在生物防治剂在综合防治项目中开发。
The eggs and second instar larvae of Spodoptera litura were treated with different concentrations of conidial suspensions of six isolates of fungi belonging to five species, Metarhizium anisopliae var. anisopliae (Metschnikov) Sorokin (ARSEF 7487), Lecanicillium muscarium (Petch) Zare W Gams (ARSEF 7037 and ARSEF 6118), Cordyceps cardinalis (ARSEF 7193), Fusarium lateritium Nees (ARSEF 8291/MTCC 9050) and Aspergillus sp. (ARSEF 8519). In bioassay with unscaled eggs, M. anisopliae, C. cardinalis, F. lateritium and Aspergillus sp. resulted in 100% mortality above 106 conidia/mL. However, with scaled egg masses, the highest hatching rate (56%) was observed with L. muscarium (ARSEF 6118) whereas the lowest hatchings were observed in the case of M. anisopliae followed by L. muscarium (ARSEF 7037), Aspergillus sp., F. lateritium, and C. cardinalis. The larvae were also found susceptible to all isolates in a dose dependent manner. Three promising isolates against larvae, viz. M. anisopliae, F. lateritium and L. muscarium (ARSEF 7037) resulted in average percent mortalities of 88, 89 and 77%, respectively at approximate to 108 conidia/mL. When both larvae and the leaves (provided as food) were treated with approximate to 108 conidia/mL, mortality further increased for these isolates. Based on the mortality data and the LC50 values, we suggest that M. anisopliae, F. lateritium and L. muscarium (ARSEF 7037) could be developed as potential biocontrol agents against rice cutworm in IPM programs.